8Hz和16Hz次声多次作用后TNF-α及IL-8的变化  

Changes of TNF-α and IL-8 in the homogenate of lungs after the 8 Hz and 16 Hz infrasound operation on the rats

作　　者：李唯[1] 田君 贾克勇[3] 李焕章[1] 倪殿涛[1] 戴艳丽[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安715400 [2]陕西韩城矿物局医院内科,陕西西安715400 [3]第四军医大学西京医院康复理疗科,陕西西安710033

出　　处：《第四军医大学学报》2002年第2期111-113,共3页Journal of the Fourth Military Medical University

基　　金：全军重点基金课题 ( No.96 2 0 42 )

摘　　要：目的　观察次声作用后肺组织匀浆中细胞因子TNF- α及 IL- 8的变化 .方法　 SD大鼠 190只随机分为正常对照组 (2组 ) ,次声作用 3,7,14,2 1次 4组 ,置第四军医大学研制的次声压力仓内致伤 .次声作用组以 8Hz,声压分别为12 0 ,130 d B和 16 Hz声压为 90 ,130 d B的强度 ,每次作用 2h,连续 2 1次 .结果　 8Hz12 0 ,130 d B次声作用 3,7,14次后 TNF- α及 IL- 8与正常对照组相比有显著差异 (P<0 .0 1) ,作用 2 1次后与正常对照组相比无显著差异 (P>0 .0 5 ) .16Hz90 ,130 d B的次声作用 3,7,14次后 TNF- α及 IL- 8与正常对照组相比有显著差异 (P<0 .0 1) ,作用 2 1次后与正常对照组相比无显著差异 (P>0 .0 5 ) .结论　 1次声的累积作用证明了细胞发生的变化性质和程度主要与次声作用的声压强度和时间 (作用次数 )有关 ;2次声作用后肺组织匀浆中TNF- α和 IL- 8形成时相性的反应过程 .这一改变可能与非炎症细胞结构和功能的原发性损伤有关 .AIM To observe the change of TNF αand IL 8 in the homogenate of lungs after infrasound operation. METHODS 190 SD rats were randomized into 6 groups: Control, infrasound damage 3, 7, 14 and 21 times. 8 Hz 120, 130 dB and 16 Hz 90, 130 dB infrasound damage was applied to infrasonic damage groups for 2 h once according to the planned schedule. RESULTS With 8 Hz 120, 130 dB to the lungs 3, 7, 14 times, the change of TNF α and IL 8 compared with that of control group had more radical changes ( P <0.01), no more radical changes in 21 times ( P >0.05). With 16 Hz 90, 130 dB to the lungs 3, 7, 14 times, the, change of TNF α and IL 8 compared with that of control group had more radical changes ( P <0.01), also no more radical changes in 21 times. CONCLUSION ① Accumulation of threshold shows that the changes are related to the times and the intensity of infrasound; ② After infrasound action, TNF α and IL 8 of lung homogenate represent changes in a time course dependent manner, which may be related to the primary affection of the structure and function of the non inflammatory cells. The lung might show adaptive action to the infrasound.

关 键 词：次声 TNF-Α IL-8 生物学效应 

分 类 号：R594.9[医药卫生—内科学]