鼠大脑中动脉肽能神经分布及免疫组织化学研究  

Peptidergic Innervation in the Middle Cerebral Artery of Rat:An Immunohistochemical Study

作　　者：邵立健[1] 冯微[2] 林雪群[1] 

机构地区：[1]江西医学院解剖学教研室,江西南昌330006 [2]江西医学院生物教研室,江西南昌330006

出　　处：《江西医学院学报》2002年第1期19-21,F002,共4页Acta Academiae Medicinae Jiangxi

基　　金：卫生部科学基金项目 (NO .98 1 337) ;江西省自然科学基金项目 (NO .0 14 0 0 2 3)

摘　　要：目的 :观察鼠大脑中动脉不同部位降钙素基因相关肽 (CGRP)能神经分布特征。方法 :应用免疫组织化学ABC法和神经交点计数法 ,观察 14只SD大鼠大脑的中动脉。结果 :大脑中动脉的近、中、末段均有黑色细线状的CGRP免疫反应阳性神经纤维 ,分布密度以近段明显为高 ,中段和末段依次降低 (P <0 .0 1) ;分布方式近、中和末段分别以环行型、网状型和纵行型为主。结论 :大脑中动脉不同部位CGRP能神经纤维有不同的分布方式和分布密度 。Objective: To study the innervation of calcitonin gene related Peptidergic (CGRP ergic) nerve in the middle cerebral arteries of rats. Methods: immunohistochemical ABC technique and counting number of nerve intersections were used for 14 SD rats. Results: The immunopositive fibers with the black linear structure were observed in the different locations of the middle cerebral artery.The type of CGRP ergic nerve was mainly circular (57.14%) in the proximal of MCA, mostly meshwork (85.71%)in the middle, and mostly longitudinal at the end (85.71%), The density of fibers was the highest in the proximal of MCA and the lowest at the end. Conclusion: Statistical analysis indicates that there is a significant difference of density of fibers between each two locations of MCA and a different distribution characteristic as well. It suggests that the regulation of CGRP ergic nerve might differ in the different location of the middle cerebral artery.

关 键 词：脑动脉 肽能神经分布 降钙素基因相关肽 免疫组织化学 实验动物 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]