人巨细胞病毒对人巨核系祖细胞体外生长的影响  

The effect of human cytomegalovirus on the formation of CFU-MK in vitro

作　　者：姚军霞[1] 宋善俊[1] 胡丽华[1] 向建平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血研所,武汉430022

出　　处：《临床血液学杂志》2002年第2期69-72,共4页Journal of Clinical Hematology

摘　　要：目的 :探讨人巨细胞病毒 (HCMV)对人巨核系祖细胞 (CFU MK)的抑制作用及其机制。方法 :采用体外半固体培养技术 ,观察 4种不同浓度HCMVAD 16 9株对CFU MK集落生长和成熟的影响 ,分别用PCR和RT PCR检测集落细胞中HCMV即刻早期 (IE)蛋白DNA与即刻早期 (IE)蛋白mRNA。结果 :HCMVAD 16 9株在体外能明显抑制CFU MK集落生长 ,3个感染组的CFU MK集落数均减少 ,与灭活病毒组比较 ,集落数减少程度分别为 2 1.6 %、33.8%、46 .3% (P <0 .0 5 ) ,显示抑制程度与病毒感染滴度呈剂量依赖关系 ;RT PCR检测发现HCMV感染组CFU MK细胞有IE蛋白mRNA的表达 ;PCR检测发现HCMV感染组CFU MK细胞中有IE蛋白DNA。结论 :HCMVAD 16 9株在体外能抑制CFU MK的分化和增殖 ,提示HCMV感染引起的血小板减少与CFU MK受HCMV抑制有关 ;并证实HCMV感染的CFUObjective:To investigate the mechanism and the suppression effect of human cytomegalovirus(HCMV) on colony forming unit-megakaryocyte(CFU-MK).Method: Semi-solid CFU-MK culture system was used to observe the effect of HCMV AD169 strain on CFU-MK growth of 18 cord blood samples.HCMV DNA and immediate early(IE) protein mRNA in CFU-MK was detected by PCR and reverse transcription-polymerase chain reaction(RT-PCR).Result: HCMV AD169 suppressed the formation of CFU-MK in vitro significantly. Compared with the group,the CFU-MK colonies decreased 21.6%, 33.8%,and 46.3%, ,respectively,in all the 3 infected groups(P< 0.05).The suppression and the titer of virus was dose-dependent. HCMV DNA was positively detected in the colony cells of viral infection group, and did the expression of HCMV IE protein mRNA.Conclusion: HCMV AD169 strain inhibited the differentiation and proliferation of CFU-MK by directly infecting their progenitors. There were early transcription of HCMV IE protein in CFU-MK infected by virus.

关 键 词：巨细胞病毒 巨核系祖细胞 聚合酶链反应 体外生长 集落细胞 PCR法 抑制作用 RT-PCR法 

分 类 号：R373.9[医药卫生—病原生物学]