SLE患者血清IL-4、IFN-γ水平测定及意义  

The level and its significance of IL-4 and IFN-γ in the serum of patients with SLE

作　　者：王美美[1] 许桦[1] 许晋[1] 季晓辉[2] 

机构地区：[1]东南大学附属中大医院风湿免疫科,江苏南京210009 [2]南京医科大学微生物学教研室,江苏南京210029

出　　处：《铁道医学》2002年第1期7-9,共3页Railway Medical Journal

摘　　要：目的　通过检测系统性红斑狼疮 (systemiclupuserythematosus ,SLE)患者血清细胞因子白细胞介素 4(interlukin 4,IL 4)、γ 干扰素 (interferon gamma ,IFN γ)水平 ,探讨T淋巴细胞亚群与SLE发病的关系。方法　采用酶联免疫吸附法(ELISA)测定SLE患者和健康志愿者血清IL 4和IFN γ水平。结果　 ( 1)SLE活动组血清IL 4水平和IL 4/IFN γ值明显升高 ,与非活动组、对照组比较有显著性差异 (P <0 .0 0 1)。 ( 2 )血清IFN γ水平 3组 (SLE活动组、非活动组和对照组 )间比较差异无显著意义 (P >0 .0 5 )。 ( 3)SLE患者激素治疗后血清IL 4水平明显降低。结论　IL 4、IFN γ作为区分CD4 T细胞亚群的指标之一 ,间接反映了Th1、Th2细胞活化状态 ;SLE患者血清IL 4水平、IL 4/IFN γ值明显升高 ,且与病情活动性相关 ;IFN γ水平无明显变化 ,说明活动性SLE患者存在Th2细胞优势活化状态 ,破坏了体内Th1/Th2正常平衡状态。血清IL 4水平可作为SLE病情活动性监测指标之一 ,并可作为疗效判断指标之一。寻找有效方法调节SLE患者血清IL 4水平 ,从而调节体内Th1/Th2平衡 ,将为治疗SLE开辟一条新途径。Objective To investigate the serum level of interlukin?4(IL?4,Th2?type cytokines) and interferon gamma(IFN γ ,Th1 type cytokines) in 45 patients with systemic lupus erythematosus(SLE) and 15 health individuals,and to explore the relationship between Th1/Th2 balance and pathogenesis of SLE.Methods Enzyme linked immunosorbant assay(ELISA) was used to measure the level of IL?4 and IFN γ in the serum.Results The level of IL?4 and IL?4/IFN γ ratio in active stage was significantly higher than that in static phase and health person( P < 0.001 ).There was no signifcant difference between SLE group and control group in level of IFN γ( P >0.05).The IL 4 dropped to very low level by steroid therapy.Conclusion The Th2 type cytokines(IL 4) is increased in active patiens with SLE,and an imbalance towards Th2 predominance may play a significant role in the pathogenesis of SLE,regulating IL 4 level may previde a new approach for the treatment of SLE.

关 键 词：系统性红斑狼疮 白细胞介素-4 Γ-干扰素 

分 类 号：R593.241[医药卫生—内科学]