单纯疱疹病毒性脑炎患者脑脊液和血清S-100B蛋白及NSE的测定  

S-100 B protein and neuron specific enolase levels in cerebrospinal fluid and blood serum in patients with herpes simplex encephalitis.

作　　者：赵钢[1] 杨毅宁[1] 粟秀初[1] 冯幼启[1] 代文[1] 

机构地区：[1]第四军医大学西京医院神经内科,西安710032

出　　处：《中国神经精神疾病杂志》2002年第2期99-102,共4页Chinese Journal of Nervous and Mental Diseases

摘　　要：目的　测定单纯疱疹病毒性脑炎 (HSE)患者脑脊液和血清中的S 1 0 0B蛋白和神经元特异性烯醇化酶(NSE)含量 ,并探讨S 1 0 0B蛋白和NSE含量对HSE患者脑损害的评估价值。方法　 4 2例HSE患者 (昏迷组 1 6例 ,无昏迷组 2 6例 )和 2 2例正常人对照组脑脊液和血清的S 1 0 0B蛋白和NSE含量 ,均采用酶联免疫吸附试验双抗体夹心法检测 ,并进行动态观察。结果　①HSE昏迷组的脑脊液和血清S 1 0 0B蛋白和NSE含量显著高于无昏迷组和对照组 (P均小于 0 0 1 ) ,无昏迷组脑脊液S 1 0 0B蛋白和NSE含量与对照组也有差异 (P <0 0 1和 <0 0 1 ) ;②脑脊液S 1 0 0B蛋白和NSE含量随HSE患者病情变化而发生相应的改变 ;③脑脊液S 1 0 0B蛋白和NSE含量分别与其各自的血清含量呈正相关。结论　脑脊液和血清的S 1 0 0B蛋白和NSE可以作为HSE患者脑损害的标志物 。Objective To investigate the concentrations of S 100 B beta protein (S 100 B protein) and neuron specific enolase(NSE) in cerebrospinal fluid (CSF) and serum in patients with herpes simplex encephalitis (HSE), and discuss their values in assessing brain insult. Methods S 100 B protein and NSE were measured dynamically by ELISA in CSF and serum from 42 patients with HSE and 22 controls. The patients were divided into two groups according to the severity of disease: coma group and non coma group. Results ①The levels of CSF S 100 B protein and NSE in coma group were higher than those in non coma group and control group ( P<0.01 ). The levels of CSF S 100 B protein and NSE in non coma group were also higher than those in control group ( P<0.01, P<0.05 ). ②There were dynamie changes of CSF S 100 B protein and NSE concentrations in HSE patients correlating with the severity of disease. ③Both CSF S 100 B protein and NSE concentrations also correlated with their serum concentrations. Conclusions S 100 B protein and NSE were related to the damage of glial and neuronal cells and could be used as CSF and serum markers of brain insult in patient with HSE.

关 键 词：单纯疱疹病毒性脑炎 S-100B蛋白 神经元特异性烯醇化酶 脑脊液 脑损害标志物 

分 类 号：R512.3[医药卫生—内科学]