苜蓿中华根瘤菌与鹰嘴豆中慢生根瘤菌原生质体的融合研究  

Study on fusion of protoplasts from Sinorhizobium meliloti and Mesorhizobium ciceri

作　　者：韦革宏[1] 朱铭莪[1] 郭杰[1] 陈文新[2] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨陵712100 [2]中国农业大学生物学院,北京100094

出　　处：《西北农林科技大学学报（自然科学版）》2002年第1期18-22,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 ( 30 0 0 0 0 5 )

摘　　要：利用原生质体融合技术 ,以链霉素和青霉素分别作为苜蓿中华根瘤菌 (Sinorhizobium meliloti)10 2 F2 8和鹰嘴豆中慢生根瘤菌 (Mesorhizobium ciceri ) USDA3383的抗药性选择标记 ,成功地获得了 10 2 F2 8和USDA3383的属间融合子。该融合子可分别在双亲寄主植物上结瘤 ,其在细胞形态、大小和蛋白质电泳图谱上与亲本菌株均有所差异。融合子与 10 2 F2 8的 DNA同源性为 90 .8%,而与 U SDA3383的 DNA同源性为 15 .2 %。Streptomycin and penicillin were regarded as the sign of resistance to antibiotics of Sinorhizobium meliloti 102F28 and Mesorhizobium ciceri USDA3383 respectively.Using the protoplast fusion technique,102F28 and USDA3383 were successfully fused.Fusion hybrid can inoculate in the leguminous of parental strains respectively.There were apparent differences between parents and fusion hybrid in cell morphology,and pattern of whole-cell protein.The values of DNA homology between fusion hybrid and 102F28 and USDA3383 were 90.8% and 15.2% respectively.

关 键 词：原生质体融合 苜蓿中华根瘤菌 鹰嘴豆中慢生根瘤菌 基因重组 属间融合 

分 类 号：Q939.114[生物学—微生物学] S154.381[农业科学—土壤学]