不同方法检测鼠疫动物脏器的研究  

Comparing of different methods for testing plague in rats tissues

作　　者：张洪英[1] 吴明寿[1] 郭英[1] 张丽云[1] 董兴齐[1] 

机构地区：[1]云南省流行病防治研究所,大理671000

出　　处：《中国地方病防治》2002年第1期27-28,共2页Chinese Journal of Control of Endemic Diseases

基　　金：云南省应用基础研究项目资助课题 (98C1 1 0M)

摘　　要：应用双重式聚合酶反应 (Pla -F1-PCR)、常规细菌培养和反相血凝试验 (RIHA) ,对 9只实验感染鼠疫的动物脏器 (豚鼠 6份 ,小白鼠 3份 ) ,在 4℃条件下保存不同时间后进行同步检测。结果表明 ,保存 90d和 180d后 ,PCR与RI HA均为阳性 ,而细菌培养阳性率明显降低 ,PCR和RIHA法敏感性高于常规培养。提示对鼠疫或疑似鼠疫材料的检测除常规培养外应做PCR和RIHA ,以防漏诊。This paper reported that the double primers(pla-F1)PCR,bacteria growth and the indiect hemagglutination test (RIHA) were applied in 9 materials of rats of plague(6 guinea pigs,3 small white rats) for different preserving times.The results showed that all samples were positive detected by PCR and RIHA methods after preserving 90 and 180 days under 4℃.The positive rate of the bacteria nurture was lower.The PCR and RIHA methods more sensitivety than growth.Suggestion,when detected the materials of plague or suspect plague,can using PCR and RIHA methods besides with the growth to avoid the diagnostics mistake.

关 键 词：鼠疫 动物脏器 PCR 聚合酶链反应 反相血凝试验 实验研究 

分 类 号：R516.8[医药卫生—内科学]