大鼠肝ADAMs种类鉴定及肝再生过程中ADAMsmRNA差异分析  

Species of ADAMs in rat liver and changes of ADAMs mRNA during liver receneration

作　　者：林俊堂[1] 李玉昌[1] 梁卫红[1] 张会勇[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002

出　　处：《河南科学》2002年第1期38-41,共4页Henan Science

基　　金：国家自然科学基金项目 (39970 36 2 );河南省生物工程重点实验室资助项目

摘　　要：以 2 / 3肝切除 (patialhepatectomy ,PH)大鼠为材料 ,利用PT -PCR技术 ,通过ADAMs通用引物初步分析了肝脏中ADAMs种类及PH后恢复 4h和 36h时ADAMsmRNA差异。结果表明 :PH后不同恢复时间ADAMsmRNA的扩增谱带没有差异 ,但扩增谱带的强弱有变化 ;cDNA克隆和测序分析表明 ,大鼠肝脏有ADAM15和ADAM 19mRNA同源序列。This article analyzed the species of ADAMs mRNA in rat liver and their changes during liver regeneration after PH using RT-PCR. The results showed that two homogenous sequences with ADAM15 and ADAM19 were checked out in rat liver; that there was no difference of ADAMs mRNA amplified bands in different individuals and sex; that the strong and the weak of the ADAMs mRNA amplified bands was changed in different recovering time after PH.

关 键 词：肝再生 ADAMS 通用引物 RT-PCR 基因克隆 cDNA mRNA 糖蛋白 大鼠肝脏 MDCS 

分 类 号：Q78[生物学—分子生物学] Q513.2