异种肝移植急性排斥反应中细胞凋亡的研究  

Apoptosis in the acute rejection of Hamster-to-Rat liver transplantation

作　　者：谈景旺[1] 张绍庚[1] 江艺[1] 杨甲梅[2] 钱光相[2] 吴孟超[2] 

机构地区：[1]南京军区福州总医院肝胆外科中心,福建福州350025 [2]第二军医大学东方肝胆外科医院,上海200433

出　　处：《中国普通外科杂志》2002年第1期33-36,共4页China Journal of General Surgery

摘　　要：目的　 观察肝移植急性排斥反应中移植肝细胞凋亡 ,并初步探讨其分子机制。 方法　 三袖套法建立肝移植急性排斥反应模型 ,普通组织及原位末端标记法检测移植肝中细胞凋亡 ,免疫组织化学方法检测移植肝中Fas -L ,TGF -β1的表达。 结果　 在肝移植急性排斥反应中 ,移植肝出现细胞凋亡 ,并表达Fas -L ,TGF -β1,随着肝移植急性排斥反应的加重 ,其细胞凋亡增多 ,Fas L ,TGF β1表达增高。结论的　 细胞凋亡作为细胞死亡的一种机制 ,存在于肝移植急性排斥反应中 ,其机制与Fas L ,TGF β1有关 。Objective To study the occurrence of apoptosis in acute rejection of hamster-to-rat liver transplantation and the molecular mechanism. Methods On the basis of establishment of hamster-to-rat orthotopic liver transplantation, the apoptosis in xenograft were observed by histology and in situ end-lebelling of fragmented DNA;the expression of Fas-L and TGF-β1 were observed by Immunohistochemistry. Rseults In acute rejection of liver xenograft, the apoptosis of hepatocyte was detectable, meanwhile, the expression of Fas-L and TGF-β1 were found. The more severe apoptosis, the more severe acute rejection , and the more expression of Fas-L and TGF-β1 . Conclusions Apoptosis as a mechanism of cell death exists in the acute rejection of liver xenograft, and it is closely related to the expression of TGF-β1 and Fas-L.

关 键 词：肝移植 免疫学 移植物排斥 细胞凋亡 异种移植 

分 类 号：R657.3[医药卫生—外科学]