低强度激光引起线粒体功能及细胞内游离Ca^(2+)的变化  

Impact of Low Power Laser on Cellular Mitochondria Function and Cytoplasmic Ca^(2+)

作　　者：姜胜利[1] 高长青[1] 顾瑛[2] 李功宋[1] 

机构地区：[1]解放军总医院心脏外科,北京市100853 [2]解放军总医院激光科,北京市100853

出　　处：《中国激光医学杂志》2002年第1期26-28,共3页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的　研究低强度激光对心肌细胞线粒体功能及细胞内游离Ca2 + 的影响 ,探讨低强度激光提高脂质体介导的心肌细胞基因转染可能的发生机制。方法　对体外培养的Wistar大鼠心肌细胞给予低强度激光照射 ,激光波长 5 10 6nm ,功率密度为 1、5、10mW cm2 ,照射时间为6 0 0、12 0 0s ,使能量密度分别为 0 6、1 2、3、6、12J cm2 。同时设对照组。照射后 4 8h采用四唑盐 (MTT)比色法和激光共聚焦成像技术 ,分别测定细胞线粒体功能及细胞内游离Ca2 + 。结果　所有照射剂量均能显著提高心肌细胞线粒体功能 ,其中以 1 2J cm2 组的效应最明显 ,其光密度 (opticaldensity ,OD)值较对照组提高了 9 39倍 (1 0 0 6± 0 0 6 9 0 10 7± 0 0 0 7,P <0 0 1) ;其余各组OD值提高了 1 4～ 2 2倍 (P <0 0 5 )。细胞内游离Ca2 + 测定 ,各实验组均显著降低 (P <0 0 5 )。结论　低强度激光对脂质体介导的心肌细胞基因转染率的提高 ,与其增强细胞线粒体的功能有关 ,也可能与降低了细胞内游离Ca2 + 的含量 ,继而促进微管的聚合 ,增强细胞骨架的运输功能有关。Objective To investigate the influence of low power laser irradiation on cellular mitochondria function and cytoplasmic Ca 2+ . Methods Cultured cardiomyocytes were irradiated by 510 6 nm laser in a dose of 0 6, 1 2, 3, 6 or 12 J/cm 2 respectively. 48 hours later, the cellular mitochondria function and cytoplasmic Ca 2+ concentration were assessed by means of MTT method and confocal microscopy respectively. Results The function of mitochondria was improved by every given dosage of irradiation, especialy in the group of 1 2 J/cm 2 (OD: 1 006±0 069/0 107±0 007, P<0 01)and the cytoplasmic Ca 2+ concentration was reduced. Conclusions The improvement of gene transfection of cardiomyocyte caused by low power laser irradiation may be related to increased mitochondria function and reduction of cytoplasmic Ca 2+ concentration, in which the first reflects cellular metabolic status and the other may manifest improved microtubule polymerization and increase of transportation of cytoskeleton.

关 键 词：低强度激光 线粒体功能 细胞内游离CA^2+ 基因转染 心肌细胞 

分 类 号：R318.51[医药卫生—生物医学工程]