弓形虫p^(22)抗原基因克隆的构建  

Cloning of P^(22) antigen gene of toxoplasma gondii

作　　者：姜淑芳[1] 王珊[2] 盛云杰[3] 王宜军[3] 张桂珍 

机构地区：[1]北京军医学院,北京100071 [2]301医院,北京100071 [3]济南医学高等专科学校,山东济南250022

出　　处：《实用医药杂志》2002年第1期39-40,共2页Practical Journal of Medicine & Pharmacy

摘　　要：目的构建弓形虫p22抗原基因克隆。方法参照文献合成引物,利用PCR技术获取P22基因片段,PCR产物经EcoRI、BamHI双酶切后,与经同样两种内切酶切割的质粒载体连接构成重组质粒PBluscript-SK-p22转化大肠杆菌DH5a,筛选鉴定重组子。结果PCR扩增及酶切分析均证实重组子含有插入的p22基因片段。结论成功的构建了弓形虫p22抗原基因克隆,为进一步选择适当的表达系统体外表达该抗原打下了必要的基础。Objective To construct the clone of P 22 antigen gene.Methods A pair of primers were syn-thesized according to the reference documents..Using PCR,the se quences of P 22 gene were amplified.The am plified gene fragments and plasmid PBluescript -SK were di gested with BamHI and EcoRI ,and then lig-ated to con struct recombi nant plasmids PBluescript -SK-P 22 .Re sult s The recombinant DNA were primarily con firmed by genetic mark er,PCR and di gestion with restriction en zymes.Conclu sion The clone of P 22 anti-gen gene has been successful ly con structed and It is essential to ex press the antigen in prop er system.

关 键 词：弓形虫 抗原 PCR 基因克隆 

分 类 号：R382.4[医药卫生—医学寄生虫学]