银杏叶提取物对周围神经损伤后运动神经元的保护作用  

Protective effect of extract of ginkgo biloba on survival of motor neurons after peripheral nerve injury

作　　者：薛锋[1] 顾玉东[1] 陈德松[1] 李继峰[1] 

机构地区：[1]复旦大学华山医院手外科,上海200040

出　　处：《中华手外科杂志》2002年第1期46-48,共3页Chinese Journal of Hand Surgery

基　　金：国家 973创伤基础研究资助项目 (G1 9990 542 0 2 )

摘　　要：目的　研究银杏叶提取物在大鼠坐骨神经损伤后对运动神经元的保护作用。方法　SD大鼠 3 6只 ,按手术先后随机平分为银杏叶提取物 (EGb 761)组和生理盐水 (SAL)组。大鼠均制成坐骨神经部分切除后结扎断端的模型 ,术后每天经腹腔分别注射EGb 761(10 0mg/kg-1·d-1)和同体积的生理盐水直到取材。术后 2、4、6周取材 ,大鼠处死后取L4～ 6节段脊髓 ,经酶组化染色后观察乙酰胆碱脂酶 (AChE)及酸性磷酸酶 (ACP)的活性变化 ;冰冻切片硫槿染色后对前角大型运动神经元计数 ;电镜观察脊髓前角运动神经元超微结构的变化。结果　与对照组相比 ,银杏叶提取物组的乙酰胆碱脂酶、酸性磷酸酶活性、损伤侧前角运动神经元数目及前角运动神经元超微结构 ,均有明显的改善。Objective To investigate the protective effect of extract of ginkgo biloba(EGb 761) on motor neurons survival in anterior horn of spinal cord after sciatic nerve injury in rats. Methods Model of partial resection and ligation of sciatic nerve was set up in 36 SD rats. They were divided randomly into two groups: extract of ginkgo biloba Egb 761 and saline group. Injection of EGb 761 (100 mg·kg -1 ·d -1 )or saline was undertaken respectively. At 2 ,4 and 6 week postoperatively, L 4 ～ 6 spinal cord was harvested after the rats were executed. The change of AChE and ACP were observed after histochemistrical examination, and the number of motor neuron counted after frozen slice and thionine dyeing, and ultrastructural changes of motor neurons in L 4 ～ 6 segments detected. Results Compared to the control group, there was marked improvement in bioactivity of AChE and ACP, changes of survival rate and ultrastructure of motor neurons in the experimental group. Conclusions EGb 761 might play certain protective role in survival of motor neurons after peripheral nerve injury.

关 键 词：周围神经损伤 神经元保护 电子显微镜 银杏叶提取物 运动神经元 动物实验 

分 类 号：R651.3[医药卫生—外科学] R287.2[医药卫生—临床医学]