肾综合征出血热病毒PS-6株和JR-C-1株抗原性的比较研究  

Comparison of the Antigenicity of HFRS Virus Strain PS-6 and JR-C-1

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作  者:韩亮[1] 惠连[1] 郝富勇[1] 王晓宇[1] 柳梅[1] 

机构地区:[1]长春生物制品研究所,长春130062

出  处:《中国预防医学杂志》2002年第1期27-29,共3页Chinese Preventive Medicine

摘  要:目的 比较肾综合征出血热(HFRS)汉坦病毒(HV)PS-6株和JR-C-1株的抗原性,选择制备疫苗的毒种。方法 应用空斑减少中和试验(PRNT)和McAb间接免疫荧光试验(IFAT),检测PS-6株和JR-C-1株HFRS HV的抗原性。结果 PS-6和JR-C-1均为Ⅰ型HV,两株病毒的免疫血清对17株国内外不同来源的HV的中和反应和76-118株汉滩病毒(HTHV)的免疫血清基本相似,但对一些病毒的中和反应效价两者有差异。与多株McAb反应结果显示,两株病毒间存在抗原决定簇的差异。结论 PS-6和JR-C-1的抗原性与76-118株HTHV基本相同,PS-6和JR-C-1之间存在抗原性和抗原决定簇的差异,两者合用抗原性会有互补作用。Objective To compare the antigenicity of HFRS virus strain PS - 6 and JR - C - 1, selecting virus seed to prepare vaccine.Methods The antigenicity of PS - 6 and JR - C - 1 were detected using plague reduction cross neutralization test (PRNT) and indirect fluorescent antibody test (IFAT) .Result Both PS-6 and JR- C- 1 are HFRS virus type I .The neutralization reaction results of their immune serum to 17 strain HFRS viruses were basic resemblance with 76 -118 virus serum. But they were different in antibody titer from some viruses.The some epitope of PS - 6 and JR - C - 1 were different when they reacted with many McAb.Conclusions The antigenicity of PS-6, JR- C- 1 and 76- 118 are about same.PS- 6 is a few different from JR- C- 1 in antigenicity and epitope. The complementary effect will emerge when PS - 6 is used together with JR - C - 1.

关 键 词:肾综合征出血热 汉坦病毒 PS-6株 JR-C-1株 抗原性 间接免疫荧光试验 

分 类 号:R392.1[医药卫生—免疫学]

 

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