神经生长因子对大鼠脊髓损伤后神经元N-甲基D-天门冬氨酸受体的影响  

Effects of Nerve Growth Factor on NMDA Receptor after Spinal Cord Injury in Rats

作　　者：曹晓建[1] 汤长华[1] 罗永湘[2] 

机构地区：[1]南京医科大学第一附属医院骨科,江苏南京210029 [2]同济医科大学同济医院骨科,湖北武汉430030

出　　处：《南京医科大学学报（自然科学版）》2002年第1期1-3,F002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目 ( 3980 0 176 )

摘　　要：目的 :探讨神经生长因子 (NGF)对损伤脊髓保护作用的分子机制。方法 :采用Allen′s法以 10g× 2 .5cm力致伤大鼠T8脊髓 ,插蛛网膜下隙导管于术后即刻、2h、4h、8h、12h、2 4h ,各注入NGF溶液 ,并与生理盐水组和正常对照组作对照。采用原位杂交法检测N 甲基 D 天门冬氨酸受体 1(NMDAR1)mRNA在脊髓中的表达。结果 :应用原位杂交法检测 ,正常组大鼠脊髓中几乎无NMDAR1mRNA表达 ,生理盐水组NMDR1mRNA表达明显增多 ,NGF组与生理盐水组相比较 ,NMDAR1mRNA表达明显减少 (P <0 .0 1)。结论 :NGF能通过抑制脊髓中N 甲基 D 天门冬氨酸受体 (NMDAR)的生成 ,抑制钙内流 ,抑制NO的生成 ,从而保护损伤的神经组织。Objective:To explore the protective mechanisms of nerve growth factor (NGF) on spinal cord injury. Methods:The model of spinal cord injury (SCI) was established according Allen′s way, briefely a 10 g×2.5 cm impact on the posterior T\-8 spinal cord of wistar rats. NGF was given to the treatment group through the subarachnoid space tube immediately, 2, 4, 8, 12, 24 hours after spinal cord injury. The expression of N methyl D asparate receptor (NMDAR) 1 mRNA in rat spinal cord was detected by in situ hybridization. Results: Few expression of NMDAR 1 mRNA was found in rat spinal cord of the normal group animals. The expression of NMDAR 1 mRNA in NGF group was much decreased compared with that in normal saline group ( P <0.01). Conclusion:NGF may protect spinal cord against injury in vivo. One of mechanisms in that NGF may prohibit NMDAR 1 mRNA expression, prevent calcium ion influx and prevent inhibit NO production after spinal cord injury.

关 键 词：脊髓损伤 神经生长因子 N-甲基-D-天门冬氨酸受体 

分 类 号：R651.2[医药卫生—外科学]