一种新的实验性脊髓损伤中枢性疼痛动物模型的建立  

The animal model of central pain in spinal injury using WADE method

作　　者：戴红[1] 肖忠新[1] 胥少汀[2] 陈迎朝[2] 张立仁[2] 谢作磊[3] 王元身[4] 戴珂[5] 

机构地区：[1]首都医科大学康复医学教研室,100054 [2]北京军区总医院骨科 [3]协和医科大学生理室 [4]首都医科大学神经科学研究所切片室 [5]北京市神经外科研究所病理室

出　　处：《中国康复医学杂志》2002年第1期18-21,共4页Chinese Journal of Rehabilitation Medicine

基　　金：北京市自然科学基金项目 ;项目号 7982 0 16

摘　　要：目的 :研究脊髓损伤 (spinalcordinjury ,SCI)中枢性疼痛 (centralpain ,CP)的发生机制以寻找治疗的根本方法。方法 :选用成年Wistar雌性大鼠 3 0只 ,分为两组 (X1,X2 组 )。用WADE法造成脊髓L2阶段的 3 0 0gcm (X1)和 2 0 0 gcm (X2 )的损伤 ,损伤前后分别在L1阶段记录刺激胫神经引起的体感诱发电位。于术前和术后 4、8、12、16、2 4h、每天用VonFreyHair等测定大鼠的对机械性轻压、轻触损伤近端躯体的痛反应阈及前足 5 0 %抬足率的阈值的变化 ,并观察大鼠自发痛的行为学变化。结果 :两组均出现了典型的急慢性痛超敏现象 (手术前后痛阈值的比较具有显著或非常显著性差异 ,P <0 .0 5— 0 .0 1)和自发痛现象 ,其中X1组与X2 组比较 ,严重自发痛 (自噬 )的发生比例更高 ,而且重复性好 ,与人类SCI的CP中以自发痛为主要痛苦的情况相一致。结论 :用WADE法造成大鼠3 0 0 gcm的SCI是重复性好且与人类SCI的CP最接近的SCI的CP的动物模型。Objective: To research the mechanism of central pain(CP) in spinal cord injury(SCI) and to look for radical curing method . Method: 30 female rats were divided into 2 groups:X 1 and X 2.Injury in L2 segment of spinal cord using 300gcm (X 1)and 200gcm (X 2)by WADE method were conducted, and SEP in L1 segment before and 15 minutes after injury were recorded. Assess pain threshold of rostral flank of trunk to injury level to mechanical gently tactile , gently pressure sense & rate of withdrawing forefoot using Von Frey Hair 4,8,12 ,16 ,24 hour and every day after operation for about 10 months. We also observe the behavour of spontaneous pain & so on. Result: Two groups exhibited typical acute and chronic allodynia phenomina (Comparison of pain threshold before & after operation was found to have significance or much signifiance difference: P <0.05 or 0.01) and spontaneous pain.The incidence of serious spontaneous pain (autotomy)was higher in X 1 than that in X 2,and which is corresponded to that in CP of SCI patients.Conclusion: 300 gcm SCI in rat by WADE method has excellent reappearance and is the closest to CP in SCI in human.Therefore ,it is an excellent animal model of CP in SCI.

关 键 词：脊髓损伤 中枢性疼痛 动物模型 WADE法 SCI CP 

分 类 号：R651.2[医药卫生—外科学]