瑞香狼毒丙酮提取物抗惊厥作用研究  

An anticonvulsant study on acetone-extract from Stellera chamaejasme L

作　　者：张美妮[1] 刘玉玺[1] 孙美珍[1] 王明正[2] 郝润喜[3] 孙晓飞[4] 

机构地区：[1]山西医科大学第一医院神经内科,030001 [2]山西医科大学药理教研室 [3]山西医科大学实验中心 [4]山西医科大学药学院

出　　处：《中国药物与临床》2002年第1期18-21,共4页Chinese Remedies & Clinics

摘　　要：目的　研究瑞香狼毒丙酮提取物 (AESC)抗惊厥作用。方法　采用五种动物惊厥模型 ,测定AESC时效关系 ,抗惊厥效应 ,并分析量效关系。结果　AESC能提高电刺激大鼠皮层惊厥阈值 ,384mg/kg灌胃和 174mg/kg腹腔注射 0 5～ 1h起效 ,2～ 3h达高峰 ,作用持续 7～ 10d ;而丙戊酸镁 15 0mg/kg腹腔注射作用虽强 ,但其维持时间仅 8h。AESC对小鼠听源性惊厥实验 (AS)、最大电休克惊厥实验 (MES)、戊四唑惊厥实验 (MET)均具有剂量依赖性对抗作用 ,其抗AS、MES、MET的ED50 分别为 10 3 0 5、12 3 83和 132 0 1mg/kg。AESC亦能拮抗大鼠海人藻酸惊厥 ,明显减少湿狗样颤抖 (WDS) (P <0 0 5 ) ,有效延长惊厥潜伏期 (P <0 0 5 )。结论　AESC对多种动物惊厥模型有效 ,是一种作用持续时间长 ,抗痫谱广的抗癫痫物质 ,其作用性质与丙戊酸镁相似。Objective To experimentally study the anticonvulsant activity of an acetone-extract form Stellera chamaejasme L.(AESC).Methods Time-effect relationship,anticonvulsant activities,and dose-effect relationship of AESC were determined by using five kinds of experimental epileptic model.Results AESC could elevate threshold for localized seizure by direct cortical stimulation in rats.AESC (348 mg/kg,ig) produced an effect 1 h,peaked 2～3 h after administration;and lasted 10 days;AESC (174 mg/kg,ip) produced an effect 0.5～1 h,peaked 2 h after administration,lasted 7 days,magnesium valproate (150 mg/kg,ip) rapidly developed,but the duration was relative brief (8 h).AESC dose-dependently antagonized audiogenic seizure (AS) (ED 50 =103.05 mg/kg),maximal electroshock seizure (MES) (ED 50 =123.83 mg/kg) and metrazol seizure test (MET) (ED 50 =132.01 mg/kg).AESC could also antagonize kainic acid-induced seizure in rats,obviously decreased the frequency of wet dog shakes (WDS),markedly prolonged seizure latency (P<0.05).Conclusion AESC has an anticonvulsant effect on various animal models;with a long duration of action,it is a beoad-spectrum antiepileptic agent;its action is like magnesium valproate′s.

关 键 词：瑞香狼毒丙酮提取物 抗惊厥药 癫痫 

分 类 号：R285[医药卫生—中药学]