红细胞停搏液的心肌保护作用及对细胞间粘附分子-1表达的影响  

Effect of erythrocyte cardioplegia on myocardial protection and intercellular adhesion molecular-1(ICAM-1)expression

作　　者：王胜洵[1] 陈生龙[1] 高岚[1] 王京生[1] 漆一伟[1] 解基严[1] 

机构地区：[1]北京大学人民医院心脏外科

出　　处：《中华胸心血管外科杂志》2002年第1期28-31,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金资助 (3 9770 72 8)

摘　　要：目的　研究红细胞停搏液的心肌保护作用及其对细胞间粘附分子 1表达的影响。方法　应用滤器去除血液中的白细胞和血小板 ,配制红细胞停搏液 ,并应用Langendorff离体心模型 ,测定心率(HR)、左室收缩压峰值 (LVSP)、左室舒张末压 (LVEDP)、左室内压最大上升速率 (dp/dtmax)、冠脉流量(CF)、肌酸激酶 (CK) ,电镜下观察组织结构的变化 ,用免疫组化方法观察粘附分子 (ICAM 1)的表达情况。结果　在HR、LVSP、dp/dtmax、CF的恢复率、组织显微结构和ICAM 1表达方面 ,红细胞停搏液组优于全血及去白细胞停搏液组 (P <0 0 5 )。Objective: Activated leukocytes and activated platelets worsen myocardial ischemic reperfusion injury. The purpose of this study is to make a new cardioplegia erythrocyte cardioplegia(EC) and to investigate the different effects of EC, blood cardioplegia(BC), and leukocyte depleted cardioplegia(DC) on myocardial protection and ICAM 1 expression. Methods: We applied a blood filter to make a new cardioplegia erythrocyte blood cardioplegia. Isolated rat hearts undergoing arrest followed by 1 hour of global ischemia and 180 minutes of reperfusion were studied using the modified Langendorff model (EC, BC, DC n=8) to observe the difference among three groups in dynamics, ICAM 1 expression via immunohistochemistry dye and myocardial ultrastructure change. Results: The difference among three groups in dynamics (HR,LVSP, dp/dt max ,CF), ICAM 1 expression on endothelial and mitochondrial structure were statistically significant and EC group was best than control groups (P<0 05). Conclusion: Experiments showed that erythrocyte cardioplegia may have better myocardial protective effects than blood cardioplegia or leukocyte depleted cardioplegia.

关 键 词：心麻痹液 再灌注损伤 胞间粘附分子 心肌保护 红细胞停搏液 

分 类 号：R654.1[医药卫生—外科学]