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作 者:高东杰[1] 康传红[1] 刘丽萍[1] 郭德栋[1] 方晓华[2]
机构地区:[1]黑龙江大学生物工程系,哈尔滨150080 [2]中国科学院发育生物学研究所,北京100080
出 处:《中国糖料》2001年第4期5-9,共5页Sugar Crops of China
基 金:国家自然科学基金资助项目(39870495
摘 要:通过栽培甜菜与白花甜菜种间杂交获真实杂种VC88-1(VVCC,2n=36),用栽培甜菜回交产生异源三倍体甜菜(VVC,2n=27),进一步用栽培甜菜回交,后代分离出带有白花甜菜染色体的栽培甜菜单体附加系完整系列(VV+1~9,2n=19)。从形态上将9种单体附加系区分为莴苣型、匍匐型、大叶型、多叶型、小叶抽薹型、长叶型、玻璃型、卷叶型及栽培型。细胞学上构成单体附加系有丝分裂中期染色体核型,测量出外加染色体长度与栽培甜菜最长染色体长度的比值(简称染色体比值),用以鉴别单体附加系类别,此比例变化在1.224~0.96之间,白花甜菜染色体是比较长的。将形态学鉴定和细胞学上的染色体鉴定结合起来,构成带有白花甜菜染色体的完整的栽培甜菜单体附加系系列。The hybrid VC88-1 VVCC 2n=36 was from hybridization between Beta vulgaris L. and B. corolliflora Zoss. and bacrossed with B. vulgaris Lthe allotriploid beet VVC 2n=27 obtained. A full set of monosomic addition lines VV+1-9C 2n=19 in B. vulgaris from B. corolliflora was produced by backcrossing the allotriploid beet VVC with B. vulgaris continuously. the nine monosomic addition lines were differentiated on their morphological type lettuce crawl big leaf more leaf small leaf and bolting long leaf grass curl leaf and cultival.Basing on the idiogram analysis in mitotio metaphase of both B. vulgaris and B. corolliflora as well as hybrid progenies the ratio of each B. corolliflora chromosomal length to the length of the longest chromosome of B. vulgaris which is called chromosomal ratio as short can be worked out. The ratio ranges between 1.224~0.96 and the chromosome of B. corolliflora are longer. Combining with the morphological classification the corolliflora chromosome can be identified on the cytological level respectively.Thusa full set of B. vulgaris monosomic addition lines with a corolliflora chromosome was constructed.
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