H-Y单抗检测方法初探  

Detection of Monoclonal Antibody Against H-Y Antigen

作　　者：牛树理[1] 刘为敏[1] 杭苏琴[1] 冯紫云[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《中国畜牧杂志》2001年第6期6-8,共3页Chinese Journal of Animal Science

摘　　要：优化间接ELISA条件 ,提高灵敏度 ,建立检测H Y抗体的检测方法。利用BALB/c公鼠免疫同系母鼠 ,制备H Y单抗。PCR验证间接免疫荧光法鉴定胚胎性别准确率。结果表明 :鉴定雄性胚胎的准确率为 83% ( 1 5 / 1 8)。鉴定雌性胚胎的准确率为 94 % ( 1 5 / 1 6)。Detection methods of monoclonal antibody against H Y antigen were established by optimized faction of indirected ELISA. After BALB/c female mice were immunized by intraperitoncal injections of syngenic male spleen cells, monoclonal antibody against H Y antigen was developed. The preimplantation mouse embryoes were used for sexing with an inderect immunofluorescence assay. Final sex of the embreyos was tested with Polymerase Chain Reaction (PCR) by amplification of Y chromosome specific sequene. The results showed that 83%(15/18) H Y positive embryos was male embryos, 94%(15/16)H Y negative embryos was female.

关 键 词：间接ELISA H-Y单抗 间接免疫荧光法 PCR 小鼠胚胎 动物免疫 

分 类 号：S852.4[农业科学—基础兽医学] S814.6[农业科学—兽医学]