人类巨细胞病毒感染对神经胶质瘤细胞HOXB5,HOXB6,HOXB7及HOXB8基因表达的影响  

Influence of human cytomegalovirus infection on the expression of HOXB5,HOXB6,HOXB7,and HOXB8 genes in gliomaous cells

作　　者：邬国军[1] 陈利玉[1] 戴橄[1] 姚孟辉[1] 陈淑贞[1] 舒明星[1] 罗敏华[1] 

机构地区：[1]中南大学湘雅医学院微生物学教研室,长沙410078

出　　处：《湖南医科大学学报》2001年第5期409-411,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助 ( 3970 0 0 0 6 )

摘　　要：以半定量RT -PCR技术检测经人类巨细胞病毒 (HCMV)感染和 /或全反式维甲酸 (ATRA)处理的神经胶质瘤细胞U2 5 1株的HOXB5 ,HOXB6 ,HOXB7和HOXB8的相对表达水平 ,以探讨HCMV致畸的可能机制。结果示 ,未经处理的U2 5 1细胞不表达HOXB5 ,HOXB6及HOXB8,但表达HOXB7;HCMV感染和 /或ATRA处理后 ,细胞仍不表达HOXB5及HOXB6 ,但在第二代时均表达HOXB8,尤以HCMV和ATRA共同处理后更明显 ;第三代后HOXB8的表达恢复正常。HCMV和 /或ATRA处理细胞后 ,HOXB7的表达在第一代即上调 ,第二代、第三代其表达稍下降 ,至第四代时表达又上调。表明HCMV致畸作用可能是通过诱导HOX基因的表达进而调节其它基因的异常表达而实现。Objective The expressions of HOXB5,HOXB6,HOXB7,and HOXB8 genes of U251 cell infected by human cytomegalovirus and/or treated with all trans-retinoic acid(ATRA) were detected by semi-quantitative RT-PCR.The results were that U251 cell did not express HOXB5,HOXB6, and HOXB8 but expressed HOXB7 without infecting HCMV and/or being treated with ATRA.After infected with HCMV and/or treated with ATRA,the expressions of HOXB7 and HOXB8 were up-regulated and the expression of HOXB7 lasted the fourth generation,while HOXB8 was up-regulated only in the second generation.These results indicate that the abnormal expression of HOXB gene induced by HCMV may play an important role in the maldevelopment.

关 键 词：HOX基因 人类巨细胞病毒 RT-PCR 神经胶质瘤 基因表达 HCMV 

分 类 号：R373.9[医药卫生—病原生物学]