不同病变关节滑膜的凋亡分析  

Analysis of synovial membrane apoptosis in different arthropathy

作　　者：邱续强[1] 

机构地区：[1]中南大学湘雅医院运动医学科,长沙410078

出　　处：《湖南医科大学学报》2001年第5期412-414,共3页Bulletin of Hunan Medical University

摘　　要：目的 :研究滑膜凋亡与不同关节病变的关系 ,并探讨病理关节软骨损害的机制。方法 :来源于手术病人关节分为正常对照组 (9例 )、骨关节炎组 (7例 )、关节脱位组 (5例 )、半月板损伤组 (5例 )以及化脓性关节炎组 (2例 )。滑膜组织学研究按常规电镜要求制片 ,美蓝染色 ,光镜观察。滑膜标本提取DNA ,并用凝胶电泳分析。结果 :DNA梯带以化脓性关节炎组最明显 ,关节脱位组次之 (但一例 2 0年病程肩关节脱位者也非常明显 ) ,骨关节炎组再次之 ,半月板损伤组最弱。对照组无可见梯带出现。结论 :病变关节滑膜凋亡的增强与关节病变的病因、病程以及病变的严重程度有关。这可能是病变关节软骨损害的机制之一。Objective To study the relationship between synovial membrane apoptosis and different arthropathy for the pathogenesis of cartilage lesion in arthropathy. Methods Synovial speciments (28 cases) were divided into control group (9 cases), osteoarthritis group (7 cases), dislocation group (5 cases), meniscus injury group (5 cases) and arthropyosis group (2 cases). Synovial histological sections were stained with methylene blue, and examined under microscope. DNA was extracted from synovial specimens, and analysed by gel electrophoresis. Results DNA ladder pattern was the most clear in the arthropyosis group, moderately clear in the dislocation group (especially in a case of 20 years-old shoulder dislocation), less clear in the osteoarthritis group, the least clear in the meniscus injury group, and was not seen in the control group. Conclusion It can be concluded that the increased apoptosis is related to causes, course and seriousity of arthropathy, and it might be a pathogenesis of cartilage lesion in arthopathy.

关 键 词：关节病 滑膜细胞 细胞凋亡 

分 类 号：R684[医药卫生—骨科学] R686.7[医药卫生—外科学]