湖南籍汉族女性HLA-A/B单倍型分析  

Analys of the HLA A/B local haplotype among the females of Han Nationality In Hunan province

作　　者：王新[1] 郭实士[2] 林秋华[1] 方小玲[1] 林力[1] 李潍[1] 梅其元[1] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011 [2]中南大学湘雅医学院免疫学研究室,长沙410078

出　　处：《湖南医科大学学报》2001年第5期431-433,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金 (项目编号 39770 771)

摘　　要：目的 :调查分析湖南籍汉族女性人群中HLA -A ,B位点单倍型基因频率。方法 :采用微量淋巴细胞毒试验法对 5 0例无血缘关系的湖南籍汉族女性HLA -A ,B位点多态性进行检查 ,通过比较连锁不平衡参数来推测单倍型。结果 :居单倍型基因频率前 3位的是A11-B6 0 ,A2 -B6 0 ,A2 4-B48,而A2 -B5 2 ,A11-B13 ,A2 4-B5 2等 3种单倍型有显著的连锁不平衡。结论 :湖南籍汉族女性HLA -A ,B位点抗原 ,特别是B抗原的分布与国内其它地区汉族人群及湖南地区汉族人群 (男女混合 )比较有其特殊性。A2 -B5 2 ,A11-B13,A2 4-B5 2三种单倍型有显著的连锁不平衡 ,可能为湖南籍汉族女性特有的单倍型。Objective To investigate and analyse HLA A/B local haplotype gene frequency (HGF) in the females of Han nationality in Hunan. Methods HLA-A, B local antigen polymorphisms were investigated via microcytotoxicity assay. Haplotype was inferred by means of linkage disequilibrium parameter. Results Among the 46 haplotypes, HGF high were: A11-B60 (HGF=0.1381), A2-B60 (HGF=0.0861), A24-B48(HGF=0.0709). Three haplotype's linkage disequilibrium parameter have significant difference, they are A2-B52, A11-B13,A24-B52. Conclusion The female of Han Nationality of Hunan HLA-A, B especially B local antigen frequency exists difference sompare with Han Nationality in Hunan and other areas in China of mix public of both sexes. When study relativity of the female disease of Han Nationality of Hunan with HAL, should notice HLA distributing particularity, be assure data veracity. Among the 46 haplotypes, A2-B52,A11-B13,A24-B52 three haplotypes exist significant linkage disequilibrium, probably they are own haplotypes at the female of Han Nationality in Hunan.

关 键 词：HLA-A抗原 HLA-B抗原 单倍型 连锁不平衡 汉族 女性 

分 类 号：R392.2[医药卫生—免疫学]