硝酸钠浓度对2株三角褐指藻生长及脂肪酸组成的影响  

Effect of NaNO_3 Concentration on the Growth and Fatty Acid Composition of Two Strains of Phaeodactylum tricornutum

作　　者：梁英[1] 麦康森[1] 孙世春[1] 

机构地区：[1]青岛海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《黄渤海海洋》2001年第4期56-62,共7页Journal of Oceanography of Huanghai & Bohai Seas

基　　金：国家海洋 86 3高新技术发展计划资助项目 ( 86 3-81 9-0 2 -0 1 )

摘　　要：在温度为 (2 2± 1 )℃ ,盐度为 2 8的条件下 ,用含不同浓度硝酸钠 (75 ,3 75 ,75 0 ,11 2 5 ,1 5 0 0 ,1 875 mg/L)的培养基对青岛海洋大学微藻种质库保存的 2株三角褐指藻(Phaeodactylum tricornutum MACC/B1 1 8,MACC/B2 2 1 )进行培养。在指数生长期末期进行收获。测定了 2株硅藻的生长及脂肪酸组成。单因子方差分析结果表明 :硝酸钠浓度对 2株三角褐指藻相对生长率和影响差异不显著 ;对脂肪酸组成的影响因种而异 ,2株硅藻的主要脂肪酸为 1 4∶ 0 ,1 6∶ 0 ,1 6∶ 1 n- 7和 2 0∶ 5 n- 3 (EPA)Growth and fath acid compositions of two strains of marine diatom Phaeodactylum tricornutum (MACC B118, MACC B221) were determined. The microalgae were grown under different initial NaNO 3 concentrations (75, 375, 750, 1 125, 1 500 and 1 875 mg/L) and harvested at late exponential growth phase. The results from one factor analysis of variance showed that the NaNO 3 concentration had not significant influence on the relative growth rate of two strains of Phaeodactylum tricornutum; the influence of NaNO 3 concentration on the fatty acid composition differed from strains to strains. The major fatty acids of two strains were 14∶0,16∶0,16∶1 n-7 and 20∶5 n-3(EPA).

关 键 词：三角褐指藻 硝酸钠 相对生长率 脂肪酸 微藻 饵料 培养液 

分 类 号：S963.213[农业科学—水产养殖]