柯萨奇B3m病毒致低硒低维生素E鼠心肌损伤的实验研究  

The Study on myocardial injury induced by CVB3m in Se and VE deficient mice

作　　者：郭玲[1] 钟学宽[1] 刘阳[1] 周令望[1] 高彦辉[1] 张淑芹[2] 曾宪惠[1] 

机构地区：[1]哈尔滨医科大学中国地方病防治研究中心克山病研究所,黑龙江哈尔滨150086 [2]哈尔滨医科大学实验动物学部,黑龙江哈尔滨150086

出　　处：《中国地方病学杂志》2001年第6期412-413,共2页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目 ( 396 70 6 0 5 );卫生部科学研究基金项目 ( 1996 -0 1-0 3)

摘　　要：目的　探讨柯萨奇病毒 (CV) B3m感染低 Se低 VE鼠心肌损伤的发病特点。方法　 1周龄乳鼠分别给予低 Se低 VE和补 Se常 VE饲料喂养 ,4周后腹腔接种病毒 ,对照组接种等量 RPMI16 40培养液 ,7d后处死 ,光镜下观察心肌的病理改变。结果　 CVB3m感染的 Se和 VE均缺乏鼠的心肌病变检出率为 93.3% ,病变为多发性大面积灶状坏死 ,融合成片 ,炎性细胞较少浸润 ,与急性克山病的病理改变类似 ;而补 Se常 VE组仅见散发间质炎性灶 ,面积较小 ,伴有大量的炎细胞 ,检出率为 16 .7%。结论　低硒低维生素 E状态下 ,柯萨奇病毒Objective To research the characteristic and mechanism of myocardial injury induced by CVB3m under Se and VE deficient condition.Methods Feeding 7 day old Kunming mice with both Se and VE deficient composite feed and normal feed for 4 weeks ,then inoculating them intraperitoneally with CVB3m ,1 week later, killing them by cervical dislocation .The myocardial lesions were observed under light microscope.Results Contrasted with the mice fed normally ,the mice fed with composite feed demonstrated severe lesions and high morbidity after virus infection, the detective ratio are 93.3% and 16.7% respectively.Conclusions Both Se and VE deficiency aggravate myocardium injury induced by Coxsackievirus B3m infection.

关 键 词：硒 维生素E 柯萨奇病毒 心肌损伤 

分 类 号：R542.3[医药卫生—心血管疾病]