博莱霉素诱导大鼠实验性肺纤维化肺泡Ⅱ型上皮细胞MMP-2和MT1-MMP mRNA表达的动态变化  

Expression of MMP-2 and MT1-MMP in type Ⅱ Pneumocytes during Rat Pulmonary Fibrosis Model Induced by Bleomycin

作　　者：卢韶华[1] 张农[1] 张秀荣 吴浩 方磊 张月娥 许祖德 

机构地区：[1]复旦大学基础医学院病理学教研室

出　　处：《复旦学报（医学版）》2001年第6期501-504,511,共5页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金(3 9770 2 94);上海市教委重点学科基金 (B980 80 2 )资助项目

摘　　要：目的　探讨肺泡Ⅱ型上皮细胞MMP 2和MT1 MMP在肺纤维化中的表达及意义。方法　气管内注射博莱霉素制备大鼠肺纤维化模型 ,在肺纤维化形成过程的不同阶段分离和提取肺泡Ⅱ型上皮细胞 ,应用RT PCR方法 ,观察其MMP 2及MT1 MMPmRNA表达的动态变化。结果　 (1)在博莱霉素作用后 1～ 7d大鼠肺泡Ⅱ型上皮细胞MT1 MMP转录水平明显增高 ,分别为对照组的 1.8和 1.9倍 ;(2 )肺纤维化早期肺泡Ⅱ型上皮细胞MMP 2转录水平也增强。结论　肺泡上皮不单是致纤维化因素的损伤对象 ,其损伤后活跃增生的肺泡Ⅱ型上皮细胞可以通过释放MT1 MMP ,催化MMP 2 ,从而引起和 (或 )Purpose To study the effects of MMP?2 and MT1?MMP expression in type Ⅱ pneumocytes during rat pulmonary fibrosis model induced by bleomycin. Methods Type Ⅱ pneumocytes were isolated in various periods of rat pulmonary fibrosis,and then the expression of MMP?2 and MT1?MMP mRNA were evaluated by RT?PCR quantitative analysis. Results (1) The expression of MMP?2 and MT1?MMP mRNA increased markedly at 1st and 7th day after bleomycin;(2) MMP?2 transcripts of alveolar type Ⅱ cells increased slightly in early stage of pulmonary fibrosis. Conclusions Type Ⅱ pneumocytes were not the only object of causative stimuli resulted in cell damage,but also involved in the development of pulmonary fibrosis by proliferation and secreting MT1?MMP,which activated pro MMP?2 and destroyed the pulmonary basement membrane afterwards.

关 键 词：肺间质纤维化 肺泡Ⅱ型上皮细胞 基质金属蛋白酶-2 膜型基质金属蛋白酶 逆转录多聚酶链反应 博莱梅素 

分 类 号：R563.9[医药卫生—呼吸系统]