心脑通抑制内皮细胞和血小板表达粘附分子  

Inhibition Effect of Xinnaotong on Endothelial Cells and Platelets Expression Adhesion Molecule in Vitro

作　　者：梁中琴[1] 陆群[1] 朱益[1] 顾振纶[1] 范盘生[1] 

机构地区：[1]苏州大学医学院药理学教研室,苏州中药研究所江苏苏州215007

出　　处：《中成药》2001年第12期894-896,共3页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助课题 (第 39870 945号 )

摘　　要：目的 :探讨心脑通对培养人脐静脉内皮细胞 (HUVEC)和血小板表达粘附分子的影响。方法 :用流式细胞仪检测肿瘤坏死因子 (TNF α)诱导人脐静脉内皮细胞ICAM I和凝血酶诱导人血小板P 选择素的表达。结果 :HUVEC经过TNF α处理后 ,ICAM I表达明显增加 ,在TNF α处理前 ,用心脑通 (0 .2 5～ 2 g/L)处理HUVEC ,则心脑通可剂量依赖性地抑制TNF α的作用。相同剂量的心脑通处理人血小板后 ,也可抑制凝血酶诱导的人血小板表达P 选择素。结论Objective:To study the action of Xinnaotong on adhesion molecule expression by cultured endothelial cells and platelets. Methods: Tumor necrosis factor α(TNF α) stimulated ICAM I expression on the cell surface was studied with human umbilical vein endothelial cells(HUVEC).Thrombin stimulated expression of platelet P selectin was studied with human blood platelets. Adhesion molecule expression was measured by flow cytometry. Results: ICAM I molecule expression on HUVEC was significantly stimulated by TNF α. The stimulatory effect of TNF α on HUVEC was inhibited by Xinnaotong(0.25～2g/L) in a concentration dependent manner. The same dose of Xinnaotong can inhibit the p selectin expression on human blood platelets stimulated by thrombin. Conclusion: Xinnaotong inhibites expression of adhesion molecues(ICAM I, p selectin) in HUVEC and in human blood platelets.

关 键 词：内皮细胞 心脑通 血小板 粘附分子 

分 类 号：R285.5[医药卫生—中药学]