以人精子抗原消化道免疫后的体液免疫反应  

THE HUMORAL IMMUNE RESPONSE OF DIFFERENT STRAINS OF MICE AFTER INTRAGASTROINTESTINAL IMMUNIZATION WITH HUMAN SPERM ANTIGENS

作　　者：陈云亮[1] 贲昆龙[1] 曹筱梅[1] 马岚[1] 商恩缘[1] 封树林 

机构地区：[1]中国科学院昆明动物研究所

出　　处：《实验生物学报》1991年第2期137-142,共6页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金;洛克菲勒人口研究基金;世界卫生组织人口部的资助

摘　　要：在Balb/c、C57和昆明鼠的消化道不同部位以人精子抗原免疫后,取其脾、肠系膜淋巴结、Peyer氏淋巴小结及子宫或附睾中的淋巴细胞进行培养。以ELISA法测定了这些部位的淋巴细胞所分泌的以及免疫小鼠肠道冲洗液和血清中的抗精子抗体。结果提示,Balb/c和昆明鼠抗精子免疫反应较强,而且雌性强于雄性。免疫部位以小肠及Peyer氏淋巴小结内注射的免疫效果较好,不仅可引起消化道局部免疫反应,而且子宫、附睾等生殖道组织也有淋巴细胞分泌抗精子抗体。The inbred Balb/c and C 57 mice, and the outbred Swiss Webster mice were intragastrointestinally immunized with human sperm antigens. The lymphocytes from the spleen, mesenteric lymph node (MLN), Peyer's patch (PP) and uterus or epididymis were isolated and cultured. The lymphocytesecreting antisperm IgG and IgA and the antisperm antibodies in the gut wash and serum were determined with enzyme-linked immunosorbent assay (ELISA).In the Balb/c and Swiss Webster mice, the immune responses to sperm have shown to be stronger than that in C 57, stronger in female than in male. The antigenicity of sperm membrane extracts seems to be higher than that of whole sperm. Antisperm antibodies secreted by lymphocytes from the epididymis and uterus have demonstrated to be detectable. For stimulation of the local immune response, the intra-PP and intralumina immunizations are more effective than others.

关 键 词：抗精子抗体 分泌性免疫 淋巴细胞 

分 类 号：R392.11[医药卫生—免疫学]