抗人血型A抗原单克隆抗体的研制  

STUDIES ON PREPARATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN BLOOD GROUP A ANTIGEN

作　　者：王润田[1] 吕占军[1] 张玉芬[1] 翟端巧 卞红磊[1] 李明湘[2] 王惠芬[1] 

机构地区：[1]河北医学院基础所免疫室 [2]河北省医学科学院微生物研究室

出　　处：《河北医学院学报》1989年第1期6-8,共3页

摘　　要：用细胞融合法获得两株稳定分泌抗人血型A抗原单克隆抗体的杂交瘤细胞株(HA_3和HA_4)。抗体特异性很好,与“O”和“B”型红细胞无交叉凝集,千余份血型测定结果与标准血清一致;抗体为IgM类,耐热、耐冻融,可望代替标准血清供市售生产。<ABSTRACT> Using cell fusion technique two hybridoma cell lines secreting specific monoclonal antibodies (McAbs)against human blood group A antigen(HA_3 andHA_4) were established. The specificity of these two McAbs is satisfactory.They didn′t agglutinate with type“O”or“B”of human blood red cells. More than a thousand human blood samples had been examined with these twoMcAbs. The results were well consistent with that of standard serum. Both HA_3 and HA_4 McAbs were of the class IgM, resistant to heat and freezingthawing. If be produced commercially, it is possible to replace the standardblood group typing serum.

关 键 词：细胞融合 杂交瘤细胞株 单克隆抗体 

分 类 号：R371.6[医药卫生—病原生物学]