去甲肾上腺素诱导左心室c-fos、c-myc基因表达  

NE induce the expression of prooncogene c-fos and c-myc in rat left ventricle

作　　者：刘晓红[1] 魏振宇[1] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003

出　　处：《遵义医学院学报》2001年第6期480-481,484,共3页Journal of Zunyi Medical University

基　　金：贵州省教育厅资助项目 (No :C - 12 6 )

摘　　要：目的　从器官水平上探讨去甲肾上腺素 (NE)诱导大鼠心肌原癌基因表达及受体机制。方法　以 10 -6MNE对大鼠离体心脏灌流 1h ,采用逆转录—多聚酶链反应 (RT PCR)方法 ,测定不同实验条件下左心室c fos和c myc表达水平。结果　 10 -6MNE灌流心脏后c fos和c myc表达均显著增强 ;用 5× 10 -6M的哌唑嗪预先灌流可完全阻断NE诱导的c fos和c myc表达增强的效应 ,而以 10 -6M的心得安预先灌流则无此作用。结论　NE经α1 受体介导 ,可诱导心肌c fos和cObjective To study the receptor mechanism of NE induced prooncogene c fos and c myc expression in rat ventricle.Methods In the study, the mRNA level of c fos and c myc in the left ventricle of the langendorff perfused rat heart were quantitated by reverse transcripition and polymerase chain reaction (RT PCR) under different experiment condition.Results NE increased the expression of c fos and c myc. The α 1 adrenergic blocker prazosin (5×10 -6 M) blocked completely the effects of NE on expression of c fos and c myc, but beta adrenergic blocker propranolol (10 -6 M) couldn't block NE induced responses.Conclusions NE can induce expression of prooncogene c fos and c myc in rat left ventricle . NE induced c fos and c myc expression is mediated by α 1 adrenergic receptor.

关 键 词：去甲肾上腺素 Α1-受体 C-FOS c-myc 大鼠 基因表达 左心室 心肌肥厚 

分 类 号：R542.2[医药卫生—心血管疾病]