免疫途径及侵袭蛋白表达对痢疾疫苗免疫效果影响的实验研究  

Immunogenicity of two different bivalent Shigellavaccines after intranasal, intragastric or intestinal administration in mice

作　　者：徐辉[1] 高杰英[1] 石辛甫[1] 邢丽[1] 彭虹[1] 舒翠莉[1] 陈志华[1] 

机构地区：[1]军事医学科学院微生物流行病研究所免疫室,北京100850

出　　处：《中华微生物学和免疫学杂志》2001年第5期527-530,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家"863"基金资助项目 ( 863 1 0 2 0 7 0 3 0 3)

摘　　要：目的　探讨免疫途径及侵袭蛋白表达对 2株痢疾疫苗免疫效果的影响。方法　FSM 2 1 1 7或FS 54 1 6以 4× 1 0 7CFU/只分别经滴鼻、灌胃、肠内注射 3种途径免疫小鼠 ,三免后 7d收集血清、小肠、鼻咽、肺、阴道冲洗液 ,ELISA法检测其中特异性福氏、宋内LPSIgA和IgG抗体 (Ab)水平。结果　滴鼻和肠内免疫都能够诱生血清中双价IgA、IgGAb显著升高 ,但仅滴鼻免疫组Ipa+株较Ipa- 株升高显著 (P <0 .0 1 )。滴鼻免疫组同时诱生鼻咽、肺、小肠内特异性抗体升高 ,尤其是生殖道冲洗液内抗体升高极为明显 ;而肠内免疫组诱生小肠、肺冲洗液内特异性抗体升高 ,但鼻咽及生殖道冲洗液内抗体未见明显升高。结论　鼻粘膜免疫不仅诱导多个粘膜部位 (特别是生殖道 )的抗体反应 ,且能诱导系统免疫反应。是一个安全有效的免疫途径。研究中发现侵袭蛋白表达在鼻粘膜部位能够显著加强系统特异性抗体生成 ,但在胃肠粘膜却无此作用。Objective To observe the effect of three different mucosal administration routes to the immunogenicity of two bivalent Shigella vaccines. Methods BALB/c mice were divided into three groups with 20 mice in each. Mice were immunized respectively with Ipa + or Ipa - vaccines(4×10 7CFU) three times with an interval of two weeks by intranasal,intragastric or intraintestinal routes. The serum, nasopharynx wash, lung wash, small intestinal wash and vaginal wash were collected. IgA and IgG antibodies against flexineri and sonnei LPS were measured by ELISA. Results The levels of IgA and IgG Ab in serum of intranasal and intraintestinal vaccinated groups were significantly increased. Significant difference were only observed in the serum IgA and IgG Ab and nasopharynx wash IgA Ab of intranasal group between Ipa + and Ipa - groups. At the same time, the levels of IgA and IgG Ab in nasopharynx wash, lung wash, small intestinal wash and vaginal wash were significantly increased after intranasal immunization with two bivalent Shigella vaccines. The levels of IgA and IgG Ab in small intestinal wash and lung wash were significantly increased after intraintestinal immunization. Conclusion Two bivalent Shigella vaccines can induce systemic and local mucosal immunity reactions by nasal or small intestinal mucosa vaccination in a low dosage compared with intragastric route(about 1/20 ). Ipa can significantly enhance the levels of serum IgA and IgG Ab in intranasal vaccinated animals.

关 键 词：痢疾疫苗 侵袭蛋白 滴鼻免疫 灌胃免疫 肠内免疫 

分 类 号：R392-33[医药卫生—免疫学]