环磷酰胺诱发永生化人支气管上皮细胞的恶性转化  

Malignant transformation of immortalized human bronchial epithelial cells induced by cyclophosphamide

作　　者：袁素波[1] 廖明阳[1] 叶常青[2] 王治乔[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《中国药理学与毒理学杂志》2001年第6期441-446,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：北京市科委实验动物专项基金资助项目( 95 4 0 2 4 5 0 0 )~~

摘　　要：研究环磷酰胺 (CP)对人类支气管上皮细胞的致癌转化效应 .实验利用永生化人支气管上皮细胞(BEAS 2B)为靶细胞模型 ,研究CP诱导的BEAS 2B细胞 (BEAS CP)的恶性转化 ,并伴随研究了BEAS CP转化细胞的增殖动力学 ,细胞周期和非贴壁依赖性生长等转化表型特征 .结果显示 ,经传代和软琼脂培养基筛选 ,BEAS CP细胞已具有较为稳定的转化表型和细胞生物学特性 ,可能是具有裸小鼠致瘤性的恶性转化细胞 .结果表明CP对BEASTo investigate the transformation effects of cyclophosphomide(CP) on human bronchial epi thelial cells, the immortalized nontumorigenic human bronchial epithelial cells(BEAS 2B) were used as a target cell model and the transformation phenotype of CP transformation cells(BEAS CP), such as dynamics of proliferation, cell cycle and anchorage independence growth, etc. were also studied at the same time. By passing generation and selection in soft agar medium, a more stable transformed phenotype and cellular biological characteristics were established in the BEAS CP cell, it seems to be a malignant transformed cell that might have the tumorigenicity in nude mice. The results indicate that CP has the malignant transformation effects on BEAS 2B cells.

关 键 词：环磷酰胺 上皮细胞 支气管 人 转化 恶性 细胞增殖动力学 

分 类 号：R99[医药卫生—毒理学]