大黄酸玻碳修饰电极对血红蛋白的催化还原  

Electrocatalysis of Hemoglobin at Rhein Modified Glassy Carbon Electrodes

作　　者：张玉忠[1] 赵红[1] 袁倬斌[1] 

机构地区：[1]中国科学技术大学研究生院化学部,北京100039

出　　处：《高等学校化学学报》2002年第3期391-393,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9875 0 2 7)资助

摘　　要：The electrochemical behavior of rhein modified glassy carbon electrodes were studied. Three peaks in CV appeared in the potential rang of +0.4\-0.8 V\%（vs\%. Ag/AgCl） in 0.06 mol/L H\-2SO\-4. p\-1 and p\-3 are a couples of oxidation\|reduction peak, the electrode process contains two electrons and two protons. The formal potential（\%E\%\+\{o\} ）is -0.202 V（\%vs\%. Ag/AgCl） at the scanning rate of 50 mV/s. The cathodic peak potential shifts linearly in the negative direction with increasing solution pH with a slope of 67 mV per pH unit. The rhein modified electrode shows an electrocatalytic activity for reduction of hemoglobin in 0.06 mol/L H\-2SO\-4 solution. The peak currents are proportional to the concentrations of the hemoglobin in the range of 6.0×10\+\{-8\}\1.3×10\+\{-6\} mol/L, the relative standard deviation is 3.5% for 6 successive determination of 1.0×10\+\{-7\} mol/L hemoglobin solution. The catalytic mechanism has been discussed.The electrochemical behavior of rhein modified glassy carbon electrodes were studied. Three peaks in CV appeared in the potential rang of +0.4\_-0.8 V\%(vs\%. Ag/AgCl) in 0.06 mol/L H\-2SO\-4. p\-1 and p\-3 are a couples of oxidation\|reduction peak, the electrode process contains two electrons and two protons. The formal potential(\%E\%\+\{o\} )is -0.202 V(\%vs\%. Ag/AgCl) at the scanning rate of 50 mV/s. The cathodic peak potential shifts linearly in the negative direction with increasing solution pH with a slope of 67 mV per pH unit. The rhein modified electrode shows an electrocatalytic activity for reduction of hemoglobin in 0.06 mol/L H\-2SO\-4 solution. The peak currents are proportional to the concentrations of the hemoglobin in the range of 6.0×10\+\{-8\}\_1.3×10\+\{-6\} mol/L, the relative standard deviation is 3.5% for 6 successive determination of 1.0×10\+\{-7\} mol/L hemoglobin solution. The catalytic mechanism has been discussed.

关 键 词：血红蛋白 大黄酸 修饰电极 电催化 催化还原 电化学分析 玻碳电极 循环伏安法 

分 类 号：Q51[生物学—生物化学] O657.1[理学—分析化学]