静脉充氧结合抗氧化剂“复苏”无心跳供肝的实验研究  

Liver transplantation with no-heatbeating donor supplied gaseous oxygen during cold preservation in pigs

作　　者：傅仲学[1] Setfan Saad Manfred Nagelschmidt 韩文妙[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,400016 [2]德国科隆大学医学院第二外科

出　　处：《中华肝脏病杂志》2002年第1期17-20,共4页Chinese Journal of Hepatology

摘　　要：目的 探讨利用静脉充氧“复苏”较长时间无心跳（热缺血 ３０～６０ ｍｉｎ）供体器官技术进行动物原位肝移植的可行性。方法 ３６头家猪随机分为供体和受体各３组，供体 ３组为 Ａ组（肝脏热缺血 １５ ｍｉｎ、Ｂ组（肝脏热缺血 ６０ ｍｉｎ）和 Ｃ组（肝脏热缺血 ６０ ｍｉｎ＋冷保存期供氧），进行动物原位肝移植，观察移植动物的５ｄ生存率、肝细胞损害、肝脏台成功能和全身炎症反应指标的变化。结果Ｂ组供肝组在移植后３ｈ内动物全部死亡，Ａ、Ｃ组动物存活至预定观察时间；Ａ、Ｃ组动物的肝细胞损害、肝脏合成功能和全身炎症反应指标的变化趋势类似，两组间差异无显著性。结论 冷保存期静脉系统供氧对较长时间热缺血损伤的供体肝脏有一定的保护和复苏作用，有可能成为利用无心跳供肝进行肝移植的辅助方法。Objective To investigate the availability of liver transplantation with non- heart beating (60 min) donors supplied gaseous oxygen by hepatic vein in cold preservation period in pigs. Methods Thirty-six pigs were randomly divided into three recipient groups and three donor groups (group A-15 min of warm ischaemia. group B-60 min of warm ischaemia and group C-60 min of warm ischaemia with gaseous oxygen by hepatic vein during cold preservation period). OTL was performed after four hours cold preservation. Postoperative 5 days survival rate of the liver metabolic, function and inflammatory reaction were observed. Results All animals in group A and C survived after reperfusion for 5 days. but animals in group B died within 3 hours postreperfusion. The damage of liver construction and function were found in group A and group B. There was no significant difference of the changes between group A and C. Conclusions Gaseous oxygen persufflation during cold preservation has important protective and resuscitative effect for liver from long-time no-heart beating donors. It was possible to become a promising method for liver transplantation with no-hearbeating donors.

关 键 词：肝移植 动物实验 无心跳供 氧气 静脉充氧 抗氧化剂 原位移植 

分 类 号：R657.3[医药卫生—外科学]