缺血再灌注期间供肝糖原水平对供肝细胞内游离钙的影响  

Effect of glycogen on calcium of donor liver during ischemia-reperfusion period

作　　者：王雨[1] 田伏洲[1] 汤礼军[1] 黄大熔[1] 李小军[1] 尹致良[1] 

机构地区：[1]成都军区总医院全军普外中心,成都610083

出　　处：《中华肝脏病杂志》2002年第1期21-24,共4页Chinese Journal of Hepatology

摘　　要：目的 研究供肝糖原含量对缺血再灌注期间肝组织ＡＴＰ及胞浆游离 Ｃａ２＋的影响。方法 供体组兔（２１只）均分为 ３组：Ａ组（禁食 ２４ ｈ）、Ｂ组（正常喂食）和 Ｃ组（正常喂食＋葡萄糖补充），运用自创的兔肝保存－再灌注模型来观察冷保存、复温和再灌注期间供肝组织中糖原含量、ＡＴＰ、肝细胞膜Ｃａ２＋ＡＴＰ酶活性及胞浆内游离Ｃａ２＋浓度的变化。结果 经上述处理，于冷保存前各组供肝糖原含量两两间差异有显著性（质量分数分别为１５．３８±２．６１，５１．８０±６．６３和６３．２３±２．７５）；且于缺血再灌注期间供肝糖原含量越高则ＡＴＰ水平越高，肝细胞膜Ｃａ２＋“ＡＴＰ酶活性也越高，胞浆内游离Ｃａ２＋浓度则越低。结论 术前提高供肝糖原含量能为肝细胞在缺血再灌注期间提供相对充足的ＡＴＰ，使肝细胞膜Ｃａ２＋ＡＴＰ酶活性能保持在较高的水平，能维持细胞内外Ｃａ２＋浓度差，防止胞浆游离Ｃａ２＋超载，对减轻供肝缺血再灌注损伤可起到一定作用。Objective To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period. Methods Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting: Group B (n=7): normal laboratory chew: Group C (n=7): normal laboratory chew plus gluose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content. ATP level, viability of Ca2+ATPase and plasmic free Ca2+ concentration ([Ca2+]i) of liver tissue were measured. Results Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca2+ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca2+ concentration was lower in groups with higher glycogen content. Conclusion Donor liver with high glycogen content can provide relatively sufficient ATP. maintain better Ca2+ATPase viability and prevent plasmic free Ca2+ concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemiareperfusion injury to the donor livers.

关 键 词：肝移植 糖原 肝缺血 再灌注损伤 ATP 钙离子 细胞内游离钙 供肝 

分 类 号：R657.3[医药卫生—外科学]