川崎病患儿血小板膜糖蛋白变化的动态观察  

Dynamic study of platelets surface glycoprotein in Kawasaki disease

作　　者：庞毅[1] 梁河涛[1] 余世禄[1] 刘晓平[1] 王荃[1] 唐芳[1] 柳宇红[1] 蒋履曾[1] 

机构地区：[1]成都市第三人民医院,610031

出　　处：《中华血液学杂志》2002年第3期134-137,共4页Chinese Journal of Hematology

基　　金：成都市卫生局科研基金资助项目 ( 9780 15 )

摘　　要：目的　通过观察血小板膜糖蛋白的动态变化了解川崎病患儿血小板活化及其活化程度。方法　用流式细胞术结合多种抗血小板膜糖蛋白单克隆抗体 (单抗 ) ,测定 2 0例川崎病患儿发病1周 (治疗前 )、2周、3周、4周时血小板膜CD4 1、CD4 2a、CD61、CD62p、CD63 的表达及阿司匹林、大剂量静脉注射丙种球蛋白 (IVIG ,1～ 2g/kg)对其表达的影响 ,观察活化血小板膜CD62p与冠状动脉损伤形成之间的关系。结果　川崎病患儿发病 4周内血小板膜糖蛋白CD4 1、CD4 2a、CD61、CD62p、CD63 的表达量高于正常对照组 (P <0 .0 5 ) ,阿司匹林、大剂量IVIG不能抑制川崎病患儿血小板膜上活化糖蛋白的高表达 ;有冠状动脉损伤的川崎病患儿活化血小板膜糖蛋白CD62p的表达量明显高于无冠状动脉损伤的川崎病患儿。结论　川崎病患儿血小板高度活化 ,血液呈高凝状态 ,血小板活化是川崎病病理生理的重要表现 ;活化血小板膜糖蛋白CD62p的明显增高可以作为川崎病患儿冠状动脉损伤的指标之一。Objective To study the activation of platelets in children with Kawasaki disease(KD) at molecular level. Methods The expression of platelet surface glycoproteins CD 41,CD 42a,CD 61,CD 62p and CD 63 in 20 KD patients was measured by flow cytometry before and at 1, 2, 3 week after treatment with aspirin and high-dose(1～2?g/kg) intravenous γ-globulin(IVIG). Results The expression of glycoprotein CD 41,CD 42a,CD 61,CD 62p and CD 63 were higher in KD group than in control group. Aspirin and IVIG could not inhibit these high expression of glycoproteins. Higher expression of CD 62p was observed in patients with coronary artery injury. Conclusion Platelets were highly activated in KD patients which may be one of the most important pathophysiological step in KD. It provided a theoretical basis for treatment of KD with antagonist of glycoprotein of platelets.Obviously increase of CD 62p can be taken as a criterion for predicting coronary artery injury in KD patients.

关 键 词：川崎病 血小板膜糖蛋白 血栓 阿司匹林 血小板活化 儿童 

分 类 号：R725.4[医药卫生—儿科]