B组链球菌Ⅲ型特异性IgG抗体母婴配对的检测及其临床意义  

Assay of type-Ⅲ specific IgG antibody against group B streptococcal capsular polysaccharides in newborn infants and their mothers and clinical significance

作　　者：钟雁[1] 吴明昌[1] 张桂荣[2] 王咏红[2] 王槿芳[2] 佟月娟[2] 杨永弘[2] 

机构地区：[1]北京儿童医院内科新生儿组,100045 [2]北京市儿科研究所微生物免疫室

出　　处：《实用儿科临床杂志》2002年第2期82-84,共3页Journal of Applied Clinical Pediatrics

基　　金：国家"九五"攻关项目(项目编号:96-904-06-07)

摘　　要：目的 研究B组链球菌（GBS）Ⅲ型特异性IgG抗体在新生儿GBS感染中的作用及其临床意义。方法 取母子双份血清,应用酶联免疫吸附试验（ELISA）进行GBSⅢ型特异性IgG抗体的检测,取子血清应用对流免疫电泳（CIE）的方法进行GBS抗原的检测。结果113例中GBS抗原阳性34例,有感染症状者25例,无感染症状者9例,GBS抗原阳性者中有感染症状者的母子抗体水平明显低于无感染症状者,有感染症状者中GBS抗原阳性者的母子抗体水平明显低于GBS抗原阴性者。结论 1.GBS型特异性IgG抗体对新生儿GBS感染有保护作用;2.GBS型特异性IgG抗体的检测可作为新生儿GBS感染的辅助诊断方法。Objective To determine the function of type-specific IgG antibody against group B streptococcal capsular polysac-charides in newborn infants with GBS infection and its clinical significance. Methods The blood sera were collected from neonates and their mothers at Beijing Children's Hospital. Type-Ⅲ specific IgG antibody against Group B Streptococcal capsular Polysaccharides was determined by enzyme-linked immunosorbent assay (ELISA) . GBS antigen was performed by countercurrent immunoelectrophoresis (CIE). Results In 113 newborn infants, 34 cases were found GBS antigen positive; 25 cases had the infected symptoms ; 9 cases had no infectious symptoms. The mean type-Ⅲ specific IgG antibody concentrations of newborn infants and their mothers were significantly lower in the infected group than those in the noninfected group, while the GBS antigen positive group was lower than the negative group too. Conclusions The type-specific anticapsular antibodies can protect neonates against invasive neonatal GBS infection. Assay of type-specific IgG antibody against group B streptococcal capsular polysaccharides is a supplementary method for diagnosing neonatal GBS infection.

关 键 词：B组链球菌 Ⅲ型特异性IgG抗体 母婴配对 新生儿 

分 类 号：R722.13[医药卫生—儿科]