甲硝唑对鸡细胞免疫和体液免疫功能的作用  

Effects of metronidazole on cellular and humoral immunity in chicks

作　　者：乔彦良[1] 盛英霞 遇欣 张妍 杨汉春[3] 郭玉璞[3] 孙爱玉 原培勋[5] 

机构地区：[1]中国人民解放军济南军区军事医学研究所,山东济南250014 [2]山东神威动物保健有限公司,山东济南250014 [3]中国农业大学动物医学院,北京海淀100094 [4]山东省安丘市畜牧局,山东安丘262100 [5]山东省畜牧兽医总站,山东济南250022

出　　处：《中国兽医杂志》2001年第8期3-7,共5页Chinese Journal of Veterinary Medicine

摘　　要：本试验观察了甲硝唑对鸡细胞免疫和体液免疫功能的作用 ,并与左旋咪唑进行了比较。结果表明 2 .5～ 40 0 m g/ kg甲硝唑可显著促进鸡外周血 T淋巴细胞增殖 ,但对 B淋巴细胞无明显影响 ;可使 CD+ 4 / CD+ 8淋巴细胞比值明显升高 ;可使鸡外周血淋巴细胞诱生 IL- 2活性明显增强 ;可使鸡体内抗颗粒性胸腺依赖性抗原 SRBC抗体滴度、抗可溶性胸腺依赖性抗原BSA抗体滴度和抗非胸腺依赖性抗原 BA抗体滴度明显升高 ;可使鸡血清总补体活性明显升高。与甲硝唑比较 ,左旋咪唑也存在着非常类似的作用 ,2 .5～ 10 m g/ kg左旋咪唑可明显促进外周血 T淋巴细胞增殖 ,可使 CD+ 4 / CD+ 8淋巴细胞比值明显升高 ;可使鸡外周血淋巴细胞诱生 IL - 2活性明显增强 ;可使鸡体内抗 SRBC抗体滴度、抗 BSA抗体滴度和抗 BA抗体滴度均明显升高 ;10 m g/ kg左旋咪唑甲硝唑可使血清补体总活性升高。上述结果表明甲硝唑和左旋咪唑两种咪唑类化合物在促进鸡细胞免疫和体液免疫可能存在着相似的作用机制。The effects of metronidazole on the cellular and humoral immunity of chicks was studied and compared with levamisol.It was shown that metronidazole at doses of 2.5～400 mg/kg could increase significantly the ConA response of chick peripheral blood lymphocyte (PBL),and the IL 2 activity of PBL induced by ConA,but could not enhance the LPS response of PBL.Metronidazole(10～400 mg/kg)could increase the ratio of CD + 4/CD + 8 T lymphocyte.Significant increase was observed in antibody titers to SRBC,BSA and Brucella abortus (BA) antigen.Metronidazole(2.5～400 mg/kg) could augment the production of complement.Levamisole had very similiar effects as metronidazole at doses 2.5～10 mg/kg and 10 mg/kg respectively.The results above have shown that imidazole compounds metronidazole and levamisole might have very similiar immunomodulatory mechanism.

关 键 词：免疫增强剂 甲硝唑 左旋咪唑 布鲁氏菌抗原 鸡 细胞免疫 体液免疫功能 

分 类 号：S858.312.4[农业科学—临床兽医学]