苦味酸-天狼星红偏振光法检测增生性瘢痕组织胶原  

Detection of collagens in hypertrophic scars by picrosirius polarization method

作　　者：黎小间[1] 雷涛[1] 高建华[1] 

机构地区：[1]第一军医大学南方医院整形外科,广东广州510515

出　　处：《第一军医大学学报》2002年第3期217-219,共3页Journal of First Military Medical University

基　　金：国家自然基金项目(39870807)

摘　　要：目的探讨增生性瘢痕组织胶原检测的简易方法。方法应用苦味酸-天狼星红偏振光法和图像分析技术对增生性瘢痕组织中胶原的含量及分布特点进行观察。结果偏振光显微镜下可见增生性瘢痕组织中含有大量的嗜酸性胶原蛋白纤维束,Ⅰ型胶原纤维为黄或红色,呈致密的条束状,显示很强的双折光性;Ⅲ型胶原纤维为绿色的细纤维,散布于Ⅰ型胶原周围,呈疏网状,显示弱的双折光。经图像分析,其中Ⅰ型胶原占(73.95±3.22)%、Ⅲ型胶原占(22.13±3.57)%。结论苦味酸-天狼猩红染色和偏振光是一种观察增生性瘢痕组织胶原类型、分布、排列与含量的较理想方法。Objective To finda simplemethodto detectthecollagensinhumanhypertrophicscars.Methods Thecontent and distributionof collagenin humanhypertrophicscarswere observedwith both conventionallightand polarization microscopycombinedwithimageanalysisof hypertrophicscarsectionsstainedwithpicrosiriusred.Results Polarization microscopyrevealedcloselypackedthickfibrilsof typeⅠcollagenfibersthat showedeitherred or yellowintense birefringenceinthehypertropictissue,whereastypeⅢcollagenfibersconsistedmainlyof greenthinfibrilwithweakgreenish birefringence.Thehypertrophicscar,as indicatedby imageanalysis,contained(73.95±3.22)%typeⅠcollagenand(21.13±3.57)%typeⅢcollagen.Conclusion Polarizationmicroscopyandsiriusredstainingis an idealmethodin analyzingthe distributionandarrangementof thecollageninhypertrophicscars.

关 键 词：苦味酸-天狼星红 偏振光法增生性瘢痕 胶原 检测 染色 

分 类 号：R622[医药卫生—整形外科]