双抗体夹心斑点金免疫渗滤法检测血吸虫循环抗原的现场应用  

FIELD APPLICATION OF DETECTING SCHISTOSOMA CIRCULATING ANTIGEN BY DOUBLE ANTIBODY SANDWICH DOT IMMUNO GOLD FILTRATION ASSAY

作　　者：张素娥[1] 汤益[1] 施晓华[1] 蒋健敏[1] 

机构地区：[1]浙江省医学科学院寄生虫病研究所,杭州310013

出　　处：《中国血吸虫病防治杂志》2002年第1期44-45,共2页Chinese Journal of Schistosomiasis Control

基　　金："九五"国家科技攻关专题基金资助;浙江省自然科学基金资助项目

摘　　要：目的　探讨双抗体夹心斑点金免疫渗滤法 (S- DIGFA)在现场的应用价值。方法　在原已建立的以抗 2 6 k Da GST基因重组蛋白多克隆抗体为捕获抗体兼测示抗体的 S- DIGFA的基础上 ,应用该法检测血吸虫病中度流行区和传播阻断地区人群的血清共 1388份 ,并以双抗体夹心EL ISA(S- EL ISA)作对照。结果　用 S- DIGFA和 S- EL ISA平行检测血吸虫病中度流行区人群血清30 0份 ,阳性检出率分别为 15 .7%和 17.3% ;两法检测血吸虫病传播阻断地区人群血清 10 88份 ,阳性检出率分别为 3.9%和 3.7%。结论　 S- DIGFA检测循环抗原可在现场扩大应用和作血清流行病学调查。Objective To explore the application value of double antibody sandwich dot immuno gold filtration assay (S DIGFA) in the field. Methods On the basis of developed S DIGFA which used anti 26 kDa GST recombinant protein polyclonal antibody as capturing and detecting antibody, 1388 sera from the population of schistosomiasis middle epidemic area and transmitting blocking area were detected by S DIGFA, and by the double antibody sandwich ELISA(S ELISA) as control. Results Three hundred sera from the population of schistisomiasis middle epidemic area were parallelly detected by S DIGFA and S ELISA. The positive rates were 15 7% and 17 3%, respectively Detecting 1088 sera from the population of transmitting blocking area, the positive rates were 3 9% and 3.7%, respectively. Conclusion The detection of circulating antigen with S DIGFA can be applied in the field, and used as an epidemiological tool for survey.

关 键 词：26kDaGST 双抗体夹心斑点金免疫渗滤法 双抗体夹心酶联免疫吸附试验 日本血吸虫 循环抗原 

分 类 号：R383.24[医药卫生—医学寄生虫学] R446.62[医药卫生—基础医学]