大鼠放射复合伤伤口中血管再生及内皮细胞凋亡研究  

Studies on angiogenesis and endothelial cell apoptosis in radiation-combined wound healing in Wistar rats

作　　者：彭瑞云[1] 王德文[1] 高亚兵[1] 熊呈琦[1] 崔雪梅[1] 秦全红[1] 杨怡[1] 王晓民[1] 

机构地区：[1]北京放射医学研究所,100850

出　　处：《中华放射医学与防护杂志》2002年第1期20-21,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：全军"九五"重点招标课题基金资助项目 ( 96Z0 0 8)

摘　　要：目的　探讨放射复合伤伤口中血管再生、内皮细胞凋亡及意义。方法　Wistar大鼠皮肤伤口以 2 5Gy60 Coγ射线局部照射 ,于伤后 2、5、10、15、2 1和 2 8d活杀取材 ,采用原位末端标记、图像分析等技术研究血管再生、内皮细胞改变。结果　单创组血管再生始于伤后 2d ,于 10d其数量达高峰 ,15d后渐减少 ,此时凋亡的内皮细胞增多 ,坏死偶见 ;伤照组血管再生始于伤后 5d ,可见内皮细胞凋亡 ,15d其数量达高峰 ,2 1d坏死和凋亡的内皮细胞均增多。结论　辐射使血管再生延迟 ,内皮细胞提早凋亡 ,高峰滞后 ;辐射使血管再生障碍是辐射延迟伤口愈合的原因之一。Objective To study the changes and significance of angiogenesis and endothelial cell apoptosis in radiation-combined wound healing. Methods Wistar rats were wounded and then local irradiation was performed immediately with a dose of 25 Gy 60 Co γ-rays in the irradiation wound group.Tissue specimens were obtained at 2,5,10,15,21 and 28 days after injury and angiogenesis and apoptosis of endothelial cells were investigated by means of LM,EM and in situ terminal labeling. Results In the nonirradiation wound group,angiogenesis began from day 2 and the capillary mumber reached its peak on day 10 and decreased on day 15 after injury when the endothelial cells occurred apoptosis and scarcely necrosis.In the irradiation wound group angiogenesis began on day 5 when endothelial cells occurred apoptosis.The capillary number reached its peak on day 15 and decreased on day 21 after injury when endothelial cells occurred both apoptosis and necrosis. Conclusions Radiation may delay the angiogenesis in wound healing,induce apoptosis of endothelial cells earlier and delay the peak of the capillary number.That radiation may delay the angiogenesis is one of reasons in delay of wound healing by radiation.;

关 键 词：伤口愈合 血管再生 放射复合伤 内皮细胞凋亡 动物实验 

分 类 号：R14[医药卫生—公共卫生与预防医学]