RP-HPLC测定化淤祛斑胶囊中黄芩苷的含量  

Determination of baicalin in Huayuquban capsulae by RP-HPLC

作　　者：徐国兵[1] 关荣才[1] 

机构地区：[1]安徽省药品检验所,安徽合肥230061

出　　处：《中国药学杂志》2002年第2期133-135,共3页Chinese Pharmaceutical Journal

摘　　要：目的　研究中药化淤祛斑胶囊中黄芩苷的定量方法。方法　样品用甲醇超声提取。Shim PackCLC ODSC18(15 0mm× 4 .6mm)色谱柱 ,流动相 :甲醇 水 冰醋酸 (5 5∶4 5∶1)。紫外检测波长 2 78nm。结果　黄芩苷在 0 .2～ 1.7μg内线性关系良好。平均回收率为 99.1% ,RSD =1.31%。结论　该方法灵敏、准确、专属性强 。OBJECTIVE: To study a method for the determination of baicalin in Huayuquban capsulae. METHODS: The samples were extracted by supersonic with methanol. RP-HPLC analysis was carried out on Shim-Pack CLC-ODS C18 column and the mixture of methanol-water-acetic acid glacial (55:45:1) was used as the mobile phase. The detection wavelength was at 278 nm. RESULTESSION: The standard curve was linear over the range of 0.2 ∼ 1.7 μg, and the average recovery was 99.1% with RSD 1.31%. CONCLUSION: The method is sensive,accurate and specific. It may be used for the quantitative determination of baicalin in Huayuquban capsulae.

关 键 词：化淤祛斑胶囊 黄芩苷 反相-高效液相色谱法 中药 含量 RP-HPLC 

分 类 号：R286.0[医药卫生—中药学]