肾舒对阿霉素肾病大鼠系膜基质的影响  

Effect of Shenshu on Mesangial Matrix of Adriamycin Nephrotic Rats

作　　者：余鸿[1] 夏阳[1] 刘广益[1] 郭勇[1] 刘敦玉[2] 

机构地区：[1]泸州医学院组胚教研室,泸州646000 [2]泸州医学院附属中医院,泸州646000

出　　处：《四川解剖学杂志》2000年第4期197-200,共4页Sichuan Journal of Anatomy

摘　　要：为了探讨肾舒对阿霉素肾病(AN)大鼠的保护作用 ,我们将 40只雄性Wistar大鼠随机分为正常对照组 (N组 )、阿霉素肾病组 (A组 )、强的松治疗组 (P组 )和肾舒治疗组 (S组 ) ,采用一次性尾静脉注射阿霉素建立 AN模型 ,对 AN大鼠分别用强的松和肾舒治疗。以自动图象分析仪测定各组肾小球系膜细胞数密度、系膜基质指数 ,对免疫组织化学反应进行半定量测定肾小球中IV型胶原 (Col )、层粘连蛋白 (L N)及纤维连接蛋白 (FN)含量。结果显示 :A组大鼠肾小球系膜基质指数、系膜细胞数密度、Col 、L N和 FN均较 N组明显增加 (P<0 .0 5 ) ;S组大鼠以上五指标均较 A组明显减少 (P<0 .0 5 ) ,而 P组大鼠与 A组比较差别不明显 (P>0 .0 5 )。提示肾舒能明显减少肾小球系膜细胞的增殖和系膜基质的合成 ,从而对 AN大鼠具有明显保护作用 ,且治疗作用优于传统强的松疗法。To study the protective role of Shenshu (SS) in adriamycin nephortic(AN) rats,40 male Wistar rats were randomly divided into four groups: the normal control group(N group),the AN group(A group),the prednisone group(P group) and the SS group(S group).The AN model was established with one injection of adriamycin through caudal vein. AN rats were treated with prednisone and SS respectively. The mesangial matrix index and numerical density of mesangial cells were assayed by automatic image analyser, and the quantity of Collegen IV(Col Ⅳ),Fibronectin(FN) and Laminin(LN) in glomerular matrix were assayed by immunohistochemistry. The results display that the mesangial matrix index, the numerical density of mesangial cells and the quantity of Col Ⅳ, FN and LN in glomeruli of A group were higher than that of N group(P<0.05), but that of S group was lower obviously than that of A group(P<0.05), and that of P group had no considerable difference(P>0.05) with that of A group. These results exhibit that SS can inhibit both the proliferation of mesangial cells and the synthesis of mesangial matrix in glomeruli, therefore it has a protective role to AN rats, and this role is better than that of prednisone.

关 键 词：肾舒 阿霉素肾病 系膜基质 强的松 

分 类 号：R96[医药卫生—药理学]