HPLC法测定银杏叶片剂中总黄酮甙的含量  

Determination of total flavonoid glycoside in Ginkgo biloba leaf tablet by HPLC

作　　者：杨如同[1] 韩福贵[1] 潘福生[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《植物资源与环境学报》2001年第4期57-58,共2页Journal of Plant Resources and Environment

摘　　要：Total flavonoid glycoside was isolated from Ginkgo biloba Linn. leaf tablet and determined by RP-HPLC using quercetin as external standard. 4.6 mm×100 mm Spherisorb ODS column, mobile phase: V (CH\-\{3\}OH) ∶ V (0.3% H\-\{3\}PO\-\{4\})=40∶60, flow rate: 1 mL/min, detection: λ=370 nm. The sample was extracted with CH\-\{3\}OH by ultrasonic extraction and hydrolyzed with 25% HCl, then sample solution was diluted accurately to 50 mL. This experiment showed that this is a simple, accurate and precise method with the result of fine linearity, average recovery is 101.1% for quercetin, and RSD is 2.44%.Total flavonoid glycoside was isolated from Ginkgo biloba Linn. leaf tablet and determined by RP-HPLC using quercetin as external standard. 4.6 mm×100 mm Spherisorb ODS column, mobile phase: V (CH\-\{3\}OH) ∶ V (0.3% H\-\{3\}PO\-\{4\})=40∶60, flow rate: 1 mL/min, detection: λ=370 nm. The sample was extracted with CH\-\{3\}OH by ultrasonic extraction and hydrolyzed with 25% HCl, then sample solution was diluted accurately to 50 mL. This experiment showed that this is a simple, accurate and precise method with the result of fine linearity, average recovery is 101.1% for quercetin, and RSD is 2.44%.

关 键 词：银杏 HPLC法 银杏叶片剂 总黄酮甙 含量 

分 类 号：R284[医药卫生—中药学] S664.3[医药卫生—中医学]