超临界CO_2流体萃取穿心莲有效成分的正交试验研究  

Study on Extraction of Active Ingredients from Andrographis paniculatal Using the Orthogonal Experiment with Supercritical Carbon Dioxide

作　　者：葛发欢[1] 林秀仙[1] 黄晓芬 史庆龙 梁宝钻 李菁 钟广华 

机构地区：[1]广州健源中药科技产业有限公司

出　　处：《中药材》2002年第2期101-102,共2页Journal of Chinese Medicinal Materials

基　　金：国家"八五"攻关项目(85-11-02-03);广州市科技计划项目(2000J01001)

摘　　要：本文应用超临界CO_2萃取技术及正交试验法对穿心莲有效成分的提取工艺进行研究,所得萃取物有效成分富集,稳定性好,指标性成分脱水穿心莲内酯和穿心莲内酯含量较高。与传统醇提法比较,该方法流程短,节约溶剂,适用于工业化大生产。超临界CO_2流体萃取的最佳工艺条件为:萃取釜压力为25MPa,温度为46℃。解析釜Ⅰ压力为6 MPa,温度为65℃;解析釜Ⅱ压力为6 MPa,温度为45℃。流量为40 kg/h。This paper studied the extraction technology of active ingredients from Andrographis paniculatal using the orthogonal experiment with supercritical CO2. The active ingredients of this extract had higher purity and more stable quality than those came from conventional extract technology. And the target ingredients, dehydrated andrographolidume and andrographolidume, had higher content. Furthermore this method had shorter technology process and saved a great deal of solvent than conventional ethanol extraction, which was exactly suitable for industrialization manufac-ture. The optimization condition was: extractor pressure 25 MPa, extractor temperature 46℃, separator I pressure 6MPa, separator I temperature 65℃, separator II pressure 6MPa, separator II temperature 45℃, CO2 rate of flow 40kg/h.

关 键 词：超临界CO2流体萃取技术 穿心莲 穿心莲内脂 脱水穿心莲内酯 有效成分 正交试验 

分 类 号：R282.71[医药卫生—中药学] R284.2[医药卫生—中医学]