钙对小麦氮同化关键酶活性的影响及其与蛋白质磷酸化的关系  

Effect of calcium on key enzymes of nitrogen assimilation and related to protein phosphorylation in wheat

作　　者：鲁翠涛[1] 李合生[2] 王学奎[2] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074 [2]华中农业大学农学系,湖北武汉430070

出　　处：《植物营养与肥料学报》2002年第1期110-114,共5页Journal of Plant Nutrition and Fertilizers

基　　金：国家自然科学基金项目 ( 396 70 432 )

摘　　要：为探讨氮素同化机理 ,小麦幼苗经溶液培养 (培养液中不含Ca2 + )至二叶一心后 ,在培养液中分别设置不同浓度的Ca2 + 、Ca2 + 专一性螯合剂EGTA以及Ca2 + 通道阻塞剂LaCl3 进行处理 (进行EGTA和LaCl3 处理时 ,培养液中Ca2 + 浓度为 1mmol/L)。结果表明 ,氮同化关键酶硝酸还原酶 (NR)、谷氨酰胺合成酶 (GS)活性同Ca2 + /CaM依赖性蛋白激酶 (Ca2 + /CaM PK)活性之间存在明显的相关性。说明钙作为第二信使所介导的信号转导中 ,蛋白质可逆磷酸化可能参与了对氮素同化的调节作用。The mechanism of nitrogen assimilation on green wheat seedlings through adding different concentration of Ca 2+ ?Ca 2+ specific chelator EGTA and blocker of Ca 2+ channel LaCl 3 in nutrient solution (when treated use of EGTA or LaCl 3, there was 1mmol/L Ca 2+ in nutrient solution) was studied. The results showed that there was significant correlation between the key enzymes of nitrogen assimilation (nitrate reductase, NR; glutamine synthetase, GS) and Ca 2+ /CaM dependent protein kinase (Ca 2+ /CaM PK).It indicated that reversible protein phosphorylation could take an important part in regulating the signal transduction pathway of calcium.

关 键 词：钙 小麦 氮同化 蛋白质磷酸化 硝酸还原酶 谷氨酰胺合成酶 酶活性 信号转导 

分 类 号：S512.101[农业科学—作物学]