一种基于适配分子的偏振荧光检测法  

A Fluorescence Polarimetric Method for the Determination of Thrombin Based on Its Interaction with Aptamer

作　　者：蔡晓坤[1] 徐顺清[1] 张志仁[1] 刘志伟[1] 鲁文红[1] 

机构地区：[1]华中科技大学同济医学院环境医学研究所,武汉430030

出　　处：《分析化学》2002年第3期352-354,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助课题 (No .3 9990 5 70 )

摘　　要：凝血酶适配分子 (aptamer)可与凝血酶以高亲和力结合 ,二者的结合可改变荧光素标记的适配分子溶液的荧光偏振强度 ,据此建立了适配分子荧光偏振法测定凝血酶的新方法。方法的线性范围是 0 .1～ 4IU/mL ;检出限为 0 .0 9IU/mL。将该方法用于人体血浆中凝血酶含量分析 。The anti-thrombin aptamer can interact with thrombin in high affinity. This interaction results in the change of the fluorescent polarization in the solution of the aptamer labeled with fluorescence. We developed a new method based on the change of the fluorescent polarization resulted from this interaction to detect thrombin. The linear range of the determination is 0.1 similar to 4 IU/mL. The detection limit for thrombin is 0.09 IU/mL. This method has been used to determine thrombin in human whole blood. The results obtained are satisfactory.

关 键 词：适配分子 荧光偏振 凝血酶 血浆 临床诊断 临床检测 

分 类 号：O657.3[理学—分析化学]