低浓度三氯乙烯对人体抗氧化物酶系统影响  

Effect of Low Concentration of TCE on Human Lipid Peroxidation and Anti-oxidation Enzyme System

作　　者：黄锦生[1] 陈惠珍[1] 吴平方[1] 孙卫[1] 

机构地区：[1]深圳市卫生防疫站,518020

出　　处：《中国公共卫生》2002年第3期282-283,共2页Chinese Journal of Public Health

摘　　要：目的　观察低浓度三氯乙烯 (TCE)接触对人体脂质过氧化和抗氧化物酶系统影响。方法　用气相色谱法测定空气中的TCE浓度 ,用分光光度法测定尿中TCA浓度 ,用试剂盒测定血清中MDA、SOD、ROS、GSH和GSH -PX。结果　空气中TCE浓度为 31 5mg/m3 ,TCE接触组SOD含量明显低于对照组 ,两者差异有显著性意义 (P <0 0 1) ,且SOD含量与TCA浓度呈负相关 (P <0 0 1) ,而接触组的TCA浓度、MDA、ROS、GSH含量以及GSH -PX活力则明显高于对照组 ,MDA、ROS、GSH含量、GSH -PX活力分别与尿中TCA浓度呈正相关。结论　接触低浓度TCE可使脂质过氧化作用加强 ,脂质过氧化产物增多。同时对机体抗氧化酶系统有刺激作用 ,使机体抗氧化物酶系统反应性升高 ,呈现一种应激状态。Objective To inspect the effects of low concentration of TCE on human lipid peroxidation and anti-oxidation enzyme system.Methods The concentration of TCE in air was determined by gas chromatography,TCA in urine was determined by spectrophotometer,MDA ,SOD,ROS,GSH and GSH-PX activity in serum detected with detection kits respectively.Results The concentration of TCE in air was 31.5mg/m 3.The level of SOD of the TCE-exposure group was significant lower than that of the control group ( P <0.01),and the levels of the TCA,MDA,ROS,GSH and the activity of GSH-PX of TCE-exposure group were higher than those of the control group.The correlation between the level of SOD and the concentration of the TCA was negative( P <0.01).The correlation between the levels of MDA,ROS,GSH, GSH-PX and the concentration of TCA was positive.Conclusion The exposure to low concentration of TCE may lead to function of lipid peroxidation enhanced,and stimulate the anti-oxidation enzyme system which activity was enhanced.

关 键 词：三氯乙烯 三氯乙酸 脂质过氧化 超氧化物歧化酶 谷胱甘肽 谷胱甘肽氧化酶 有机溶剂 毒理 

分 类 号：R996[医药卫生—毒理学]