天麻阿胶联合对染铅鼠脑一氧化氮及学习记忆的影响  

Joint Effects of Gastrodia Elata Blume and Donkey-hide Gelatin on Decrease of Nitric Oxide in Cerebellum and Impairment of Learning and Memory Induced by Taking Lead in Rats

作　　者：李茂进[1] 胡俊峰[1] 张春玲[1] 于素芳[1] 韩惠芬[1] 

机构地区：[1]山东大学公共卫生学院劳动卫生与环境卫生研究所,济南250012

出　　处：《中国公共卫生》2002年第3期284-286,共3页Chinese Journal of Public Health

基　　金：山东省科委博士基金资助项目

摘　　要：目的　观察天麻和阿胶联合对染铅大鼠学习记忆损害的拮抗作用。方法　Wistar雄性大鼠经口染铅并分别单独及联合给予天麻 (4 0 g·kg-1·d-1)和阿胶 (1 0 g·kg-1·d-1)灌胃 ,连续 3个月 ;每月用游泳试验 (水中寻找平台试验 )测试学习记忆 1次 ,最后处死大鼠测定小脑组织一氧化氮含量。结果　在游泳试验中 ,高铅对照组和低铅对照组大鼠 15次中直线到达平台次数 ,与空白组比较差异均非常显著 (P <0 0 1)。天麻单用即可显著增加染铅大鼠直线到达平台的次数 (第 1月P <0 0 5 ,第 2 ,3月P <0 0 1) ;阿胶单用亦可显著增加染铅大鼠在游泳试验中直线到达平台次数 (P <0 0 5 ) ;天麻和阿胶联用更能显著增加染铅大鼠在游泳试验中直线到达平台次数 ,与二者单用相比差异非常显著 (P <0 0 1)。小脑组织一氧化氮测定 :高铅对照组和低铅对照组大鼠小脑一氧化氮 ,与空白组比较差异均非常显著(P <0 0 1) ;天麻单用即可显著提高染铅大鼠小脑一氧化氮 (P <0 0 5 ,低铅时P <0 0 1) ;阿胶单用也可显著提高染铅大鼠小脑一氧化氮 (P <0 0 5 ) ;天麻和阿胶联用更能显著提高染铅大鼠小脑一氧化氮含量 ,与二者单用相比差异非常显著 (P <0 0 1)。结论　天麻和阿胶单用即可显著减轻铅对学习记忆的损害作用 。Objective To observe and explore the antagonism of gastrodia elata blume(GTEB)and donkey-hide gelatin(DHG)combined against the impairment of learning and memory induced by taking lead in rats.Methods Wistar male rats were monopolizedly and jointly given GTEB(4 0g·kg -1 ·d -1 )and DHG(1 0g·kg -1 ·d -1 )after being poinsoned with lead by mouth everyday for three months.The capacity of learning and memory of each group was measured by swimming test every month.In the end,the rats were decapitated and nitric oxide(NO) in cerebellum tissue was measured immediately.Results In swimming test(seeking for platform or anchor in water),the decrease of number of lead controls directly seeking for anchor was high significantly compared with the blank control( P <0 01).GTEB alone did significantly increase the number of lead taking rats directly seeking for anchor compared with the lead controls( P <0 05;the second and third month, P < 01).DHG monopolized did also significantly increase the number of seeking for anchor( P <0 05).The increase of number of lead taking rats seeking for anchor induced by GTEB and DHG combined was much more significant than that induced by GTEB and DHG monopolized( P <0 01).The NO in cerebellum of lead taking rats was significantly decreased compared with the blank control.The NO in cerebellum of lead exposed rats was significantly increased by GTEB monopolized compared to lead controls ( P <0 05;the low lead leves P <0 01).DHG alone did also significantly increase NO of cerebellum of lead exposed rats( P <0 05).The increase of NO in lead exposed rats induced by GTEB and DHG combined was much more significant than that induced by GTEB and DHG monopolized( P <0 01).Conclusion The impairment of learning and memory induced by lead is significantly improved by GTEB and DHG monopolized,but the effects of GTEB and DHG combined on that was more significant.

关 键 词：铅 天麻 阿胶 学习记忆 一氧化氮 动物实验 

分 类 号：R338.64[医药卫生—人体生理学] R282.710.5[医药卫生—基础医学]