铅对幼鼠脑海马区Bcl-2、Fas蛋白表达的影响  

Effeet on Bcl-2、Fas Protein Expression in Hippocampus of Rat Offsprings Exposed to Lead

作　　者：逯晓波[1] 郑丽舒[1] 吕相征[1] 李北利[1] 蔡原[1] 陈葆春 

机构地区：[1]中国医科大学公共卫生学院 [2]辽宁省卫生监督所

出　　处：《中国公共卫生》2002年第3期291-292,共2页Chinese Journal of Public Health

摘　　要：目的　通过大鼠孕期及哺乳期不同剂量染铅所致幼鼠染铅模型 ,测定出生后 7天、2 1天幼鼠血铅、脑铅含量 ;同时测定脑组织海马区细胞凋亡相关基因Bcl- 2、Fas蛋白表达情况。方法　血铅、脑铅测定采用原子吸收石墨炉法 ;Bcl- 2、Fas蛋白表达采用免疫组化法测定。结果　对于出生后 7天幼鼠 ,染铅组与对照组相比 ,其脑组织Bcl- 2蛋白表达降低 ,而Fas蛋白表达升高 ;但对于出生后 2 1天幼鼠 ,则没有这种改变。结论　染铅可能通过改变发育期脑组织细胞凋亡相关基因Bcl- 2、Fas蛋白表达使细胞凋亡数量改变 ,从而导致铅的发育神经毒性目的　通过大鼠孕期及哺乳期不同剂量染铅所致幼鼠染铅模型 ,测定出生后 7天、2 1天幼鼠血铅、脑铅含量 ;同时测定脑组织海马区细胞凋亡相关基因Bcl- 2、Fas蛋白表达情况。方法　血铅、脑铅测定采用原子吸收石墨炉法 ;Bcl- 2、Fas蛋白表达采用免疫组化法测定。结果　对于出生后 7天幼鼠 ,染铅组与对照组相比 ,其脑组织Bcl- 2蛋白表达降低 ,而Fas蛋白表达升高 ;但对于出生后 2 1天幼鼠 ,则没有这种改变。结论　染铅可能通过改变发育期脑组织细胞凋亡相关基因Bcl- 2、Fas蛋白表达使细胞凋亡数量改变 ,从而导致铅的发育神经毒性。Objective Rat offsprings exposed to lead during pregnant and breast-feed period were used to determine the lead content in blood and brain of the 7th or 21st day after birth.Bcl-2 and Fas protein expression in hippocampus was also determined.Methods Pb concentration in blood and brain was determined by atomic absorption spectrophotometry.Bcl-2?Fas protein expression were estimated by immunohistochemical study.Results For the offsprings on the 7th day after birth,Bcl-2 protein expression in Pb-exposed groups decreased compared with the control ,and Fas protein expression increased at the same time.On the contrary,for the offsprings on the 21st day after birth,there was no such change as that in the 7th day offsprings.Conclusion Lead can induce excessive apoptosis through changing the protein expression of Bcl-2 and Fas,and then induce its developmental neurotoxicity.

关 键 词：铅 发育神经毒性 细胞凋亡 BCL-2蛋白 FAS蛋白 

分 类 号：R135.11[医药卫生—劳动卫生] R994.6[医药卫生—公共卫生与预防医学]