尿液中草酸、枸橼酸和尿酸测定方法的研究  

Study on Method of Determination of Urinary Oxalate,Citrate and Uric Acid

作　　者：张慧敏[1] 张顺祥[1] 刘桂华[1] 谢健滨[1] 沈珉[2] 

机构地区：[1]深圳市卫生防疫站,518020 [2]同济医科大学

出　　处：《中国公共卫生》2002年第3期347-349,共3页Chinese Journal of Public Health

摘　　要：目的　探讨毛细管电泳分析法同时测定尿液中草酸、构橼酸和尿酸含量的可行性。方法　选取 6 0cm× 75 μm规格的毛细管 ,4 0mmol/L的Na2 HPO4和 2 0mmol/L的硼砂配成的混合溶液作为背景电解质 ,加入 0 5mmol/L电渗流改性剂 ,在恒压为 - 15kV、恒温为 2 5℃、波长为 185nm紫外检测器等条件下 ,采取负极进样 ,阳极检测 ,进行方法的定量线性范围、灵敏度、准确度及检出限等试验。结果　在浓度范围分别为草酸 1 0mg/L～ 5 0mg/L ,柠檬酸 1 0mg/L～10 0mg/L ,尿酸 1 0mg/L～ 2 0 0mg/L内 ,3种物质浓度与峰面积的相关系数均在 0 999以上 ,出峰时间的变异系数 <1% ,峰面积的变异系数 <4 %。结论　所建立的分析方法准确度和精密度都符合分析方法的要求 。Objective To discuss and determine the contents of oxalate?citrate and uric acid in urine at the same time by capillary electrophoresis.Methods Sample was separated and the three substances in urinary were determined in 60cm×75μm capillary and phosphate/borate buffer ,additive electroomotic flow modifier at constant voltage of -15kV,constant temperature of 25℃ and 185nm wave length ultraviolet detector.Then quantitative linearity range,sensitivity,accuracy and detection limit were detected by negative pole injection and positive pole detection.Results The correlation coefficient was larger than 0.999,variation coefficient of peak time and peak area was smaller than 1%,4% in the concentration range of 1 0mg/L-50mg/L,1.0mg/L-100mg/L,1.0mg/L-200mg/L correspondingly oxalate,citrate and uric acid respectively.Conclusion The analysis method was high practical for its simplicity,rapidity,cheapness,automation and its accuracy and precision can meet the analysis requirements.

关 键 词：毛细管电泳 草酸 枸橼酸 尿酸 尿液 测定方法 

分 类 号：R446.12[医药卫生—诊断学]